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Infection and Immunity, October 2002, p. 5540-5546, Vol. 70, No. 10
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.10.5540-5546.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Effect of omp10 or omp19 Deletion on Brucella abortus Outer Membrane Properties and Virulence in Mice

Anne Tibor,1* Valérie Wansard,1 Valery Bielartz,1 Rose-May Delrue,1 Isabelle Danese,1 Patrick Michel,2 Karl Walravens,2 Jacques Godfroid,2 and Jean-Jacques Letesson1

Unité de Recherche en Biologie Moléculaire, Facultés Universitaires Notre-Dame de la Paix, B-5000 Namur,1 Centre d'Etude et de Recherches Vétérinaires et Agronomiques, B-1180 Brussels, Belgium2

Received 19 June 2001/ Returned for modification 2 August 2001/ Accepted 27 June 2002

The distinctive properties of Brucella outer membrane have been considered to be critical for Brucella sp. virulence. Among the outer membrane molecules possibly related to these properties, Omp10 and Omp19 are immunoreactive outer membrane lipoproteins. Moreover, these proteins of Brucella could constitute a new family of outer membrane proteins specifically encountered in the family Rhizobiaceae. We evaluated the impact of omp10 or omp19 deletion on Brucella abortus outer membrane properties and virulence in mice. The omp10 mutant was dramatically attenuated for survival in mice and was defective for growth in minimal medium but was not impaired in intracellular growth in vitro, nor does it display clear modification of the outer membrane properties. Significantly fewer brucellae were recovered from the spleens of mice infected with the omp19 mutant than from those of mice infected with the parent strain at 4 and 8 weeks postinfection. The omp19 mutant exhibited an increase in sensitivity to the polycation polymyxin B and to sodium deoxycholate. These results indicate that inactivation of the omp19 gene alters the outer membrane properties of B. abortus.

* Corresponding author. Mailing address: Unité de Recherche en Biologie Moléculaire, Facultés Universitaires Notre-Dame de la Paix, rue de Bruxelles 61, B-5000 Namur, Belgium. Phone: 32 81 72 44 03. Fax: 32 81 72 42 97. E-mail: anne.tibor{at}fundp.ac.be.

Editor: R. N. Moore

Infection and Immunity, October 2002, p. 5540-5546, Vol. 70, No. 10
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.10.5540-5546.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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