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Infection and Immunity, October 2002, p. 5651-5658, Vol. 70, No. 10
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.10.5651-5658.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Interleukin-4 Receptor Chain and STAT6 Signaling Inhibit Gamma Interferon but Not Th2 Cytokine Expression within Schistosome Granulomas

Ahmed Metwali, Arthur Blum, David E. Elliott, and Joel V. Weinstock^*

Division of Gastroenterology-Hepatology, Department of Internal Medicine, University of Iowa, Iowa City, Iowa 52242

Received 5 April 2002/ Returned for modification 3 June 2002/ Accepted 20 June 2002

Compared to wild-type (WT) mice, schistosome granulomas in Stat6 knockout (KO) mice lacked eosinophils and had Th1 features. Interleukin-4 (IL-4) acts through Stat6 in assisting Th2 cell development. The importance of Stat6 for Th2-cell development within schistosome granulomas had not been explored. Therefore we studied gamma interferon (IFN-), IL-4, and IL-5 production in granulomas from Stat6 KO and WT mice. Dispersed granuloma cells from Stat6 KO and WT mice made similar amounts of IL-4 and IL-5. Only Stat6 KO granuloma cells released IFN-. Granuloma T cells contained most of the IL-4, IL-5, and IFN- mRNA and secreted these cytokines. In Stat6 KO mice, 16.6% of the granuloma cells were CD4⁺. Of these, 10.7% stained for IFN- and/or IL-4 by intracytoplasmic flow analysis. Few CD4^- T cells stained positively. The IL-4-producing T cells did not stain for DX5 or with labeled -GalCer CD1d tetramer, suggesting an absence of NK T cells. Thus, conventional Th cells in Stat6 KO granulomas produce IFN- and Th2 cytokines. Stat6 limits IFN- production but is unnecessary for Th2-cell development or localization within the granuloma.

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* Corresponding author. Mailing address: Department of Medicine, 4607 JCP, University of Iowa, 200 Hawkins Dr., Iowa City, IA 52242. Phone: (319) 356-3127. Fax: (319) 353-6399. E-mail: joel-weinstock{at}uiowa.edu.

Editor: J. M. Mansfield

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Infection and Immunity, October 2002, p. 5651-5658, Vol. 70, No. 10
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.10.5651-5658.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

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