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Infection and Immunity, October 2002, p. 5670-5675, Vol. 70, No. 10
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.10.5670-5675.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Genetic Analysis of a Cryptosporidium parvum Human Genotype 1 Isolate Passaged through Different Host Species

D. E. Akiyoshi,* X. Feng, M. A. Buckholt, G. Widmer, and S. Tzipori

Division of Infectious Diseases, Tufts University School of Veterinary Medicine, North Grafton, Massachusetts 01536

Received 3 May 2002/ Returned for modification 20 June 2002/ Accepted 28 June 2002

Cryptosporidium parvum TU502, a genotype 1 isolate of human origin, was passaged through three different mammalian hosts, including humans, pigs, and calves. It was confirmed to be genotype 1 by PCR-restriction fragment length polymorphism analysis of the Cryptosporidium oocyst wall protein gene, direct sequencing of PCR fragments of the small subunit rRNA and ß-tubulin genes, and microsatellite analysis. This isolate was shown to be genetically stable when passaged through the three mammalian species, with no evidence of the emergence of new subpopulations as observed by a genotype-specific PCR assay. TU502 oocysts from different sources failed to infect gamma interferon knockout mice, a characteristic of genotype 1 isolates. The genotypic and phenotypic characterization of TU502 is significant since it is the isolate selected to sequence the genome of C. parvum genotype 1 and is currently used in several research projects including human volunteer studies.

* Corresponding author. Mailing address: Tufts University School of Veterinary Medicine, 200 Westboro Rd., Bldg. 20, North Grafton, MA 01536. Phone: (508) 839-7935. Fax: (508) 839-7911. E-mail: donna.akiyoshi{at}tufts.edu.

Editor: W. A. Petri, Jr.

Infection and Immunity, October 2002, p. 5670-5675, Vol. 70, No. 10
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.10.5670-5675.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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