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Infection and Immunity, October 2002, p. 5779-5789, Vol. 70, No. 10
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.10.5779-5789.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Variation in the Expression of a Plasmodium falciparum Protein Family Implicated in Erythrocyte Invasion

Helen M. Taylor,* Munira Grainger, and Anthony A. Holder

National Institute for Medical Research, Mill Hill, London NW7 1AA, United Kingdom

Received 1 March 2002/ Returned for modification 20 May 2002/ Accepted 11 June 2002

The PfRH protein family of Plasmodium falciparum is implicated in erythrocyte invasion. Here we report variations in the sequence, transcription, and protein expression of four different members of this family in three parasite lines, 3D7, T996, and FCB1. There are sequence polymorphisms in PfRH1, PfRH2a, PfRH2b, and PfRH3, ranging from variations across repeat regions to a 585-bp deletion in the 3' end of PfRH2b in T996. Not all the genes are transcribed: although all members of the family are transcribed in 3D7 and T996, PfRH2a and PfRH2b are not transcribed in FCB1. The PfRH1, PfRH2a, and PfRH2b proteins are expressed in late schizonts and merozoites and are located in apical organelles and on the apical surface. However, the PfRH1 protein does not appear to be correctly targeted to the apex in 3D7 and T996. In contrast, the PfRH1 protein is present at the apical end of FCB1 merozoites, but the PfRH2a and PfRH2b proteins are undetectable. The apparent redundancy in the PfRH family of proteins at the level of gene number and sequence and the variations in transcription and protein expression may allow the parasite to use alternative invasion pathways.

* Corresponding author. Mailing address: Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA, United Kingdom. Phone: 44 208 959 3666. Fax: 44 208 913 8593. E-mail: htaylor{at}nimr.mrc.ac.uk.

Editor: J. M. Mansfield

Infection and Immunity, October 2002, p. 5779-5789, Vol. 70, No. 10
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.10.5779-5789.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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