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Infection and Immunity, October 2002, p. 5870-5872, Vol. 70, No. 10
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.10.5870-5872.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Macrophage-Enhanced Germination of Bacillus anthracis Endospores Requires gerS

John A. W. Ireland1,2,{dagger} and Philip C. Hanna1*

Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109,1 Department of Microbiology, Duke University Medical Center, Durham, North Carolina 277102

Received 3 May 2002/ Returned for modification 20 June 2002/ Accepted 10 July 2002

Germination of Bacillus anthracis Sterne and plasmidless {Delta}-Sterne endospores was dramatically enhanced in RAW264.7 macrophage-like cells, while germination of nonpathogenic Bacillus endospores was not. Elimination of gerS, a germinant receptor locus, caused a complete loss of cell-enhanced germination, implicating gerS in the breaking of endospore dormancy in vivo.

* Corresponding author. Mailing address: Department of Microbiology & Immunology, University of Michigan Medical School, 5641 Med. Sci. II, Box 0620, Ann Arbor, MI 48109. Phone: (734) 615-3706. Fax: (734) 764-3562. E-mail: pchanna{at}umich.edu.

Editor: J. T. Barbieri

{dagger} Present address: Department of Biology, University of Michigan, Ann Arbor, MI 48109.

Infection and Immunity, October 2002, p. 5870-5872, Vol. 70, No. 10
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.10.5870-5872.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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