This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sun, S. Articles by Munson, R. S. Search for Related Content PubMed PubMed Citation Articles by Sun, S. Articles by Munson, R. S., Jr.

 Previous Article  |  Next Article 

Infection and Immunity, October 2002, p. 5887-5892, Vol. 70, No. 10
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.10.5887-5892.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Identification and Characterization of the N-Acetylglucosamine Glycosyltransferase Gene of Haemophilus ducreyi

Columbus Children's Research Institute,¹ Department of Pediatrics,⁵ Department of Molecular Virology, Immunology, and Medical Genetics, The Ohio State University, Columbus, Ohio 43205,² Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143,³ Buck Institute for Age Research, Novato, California 94945⁴

Received 12 April 2002/ Returned for modification 13 May 2002/ Accepted 3 July 2002

Haemophilus ducreyi is the causative agent of chancroid, a sexually transmitted ulcerative disease. In the present study, the Neisseria gonorrhoeae lgtA lipooligosaccharide glycosyltransferase gene was used to identify a homologue in the genome of H. ducreyi. The putative H. ducreyi glycosyltransferase gene (designated lgtA) was cloned and insertionally inactivated, and an isogenic mutant was constructed. Structural studies demonstrated that the lipooligosaccharide isolated from the mutant strain lacked N-acetylglucosamine and distal sugars found in the lipooligosaccharide produced by the parental strain. The isogenic mutant was transformed with a recombinant plasmid containing the putative glycosyltransferase gene. This strain produced the lipooligosaccharide glycoforms produced by the parental strain, confirming that the lgtA gene encodes the N-acetylglucosamine glycosyltransferase.

Editor: B. B. Finlay

This article has been cited by other articles:

• Post, D. M. B., Munson, R. S. Jr., Baker, B., Zhong, H., Bozue, J. A., Gibson, B. W. (2007). Identification of Genes Involved in the Expression of Atypical Lipooligosaccharide Structures from a Second Class of Haemophilus ducreyi. Infect. Immun. 75: 113-121 [Abstract] [Full Text]  
• Post, D. M. B., Mungur, R., Gibson, B. W., Munson, R. S. Jr. (2005). Identification of a Novel Sialic Acid Transporter in Haemophilus ducreyi. Infect. Immun. 73: 6727-6735 [Abstract] [Full Text]  
• Hiratsuka, K., Logan, S. M., Conlan, J. W., Chandan, V., Aubry, A., Smirnova, N., Ulrichsen, H., Chan, K. H. N., Griffith, D. W., Harrison, B. A., Li, J., Altman, E. (2005). Identification of a D-glycero-D-manno-Heptosyltransferase Gene from Helicobacter pylori. J. Bacteriol. 187: 5156-5165 [Abstract] [Full Text]  
• Vakevainen, M., Greenberg, S., Hansen, E. J. (2003). Inhibition of Phagocytosis by Haemophilus ducreyi Requires Expression of the LspA1 and LspA2 Proteins. Infect. Immun. 71: 5994-6003 [Abstract] [Full Text]