This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mukherjee, J.
Right arrow Articles by Tzipori, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mukherjee, J.
Right arrow Articles by Tzipori, S.

 Previous Article  |  Next Article 

Infection and Immunity, October 2002, p. 5896-5899, Vol. 70, No. 10
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.10.5896-5899.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Production and Characterization of Protective Human Antibodies against Shiga Toxin 1

Jean Mukherjee,1 Kerry Chios,1 Dianne Fishwild,2 Deborah Hudson,2 Susan O'Donnell,2 Stephen M. Rich,1 Arthur Donohue-Rolfe,1 and Saul Tzipori1*

Division of Infectious Diseases, Tufts University School of Veterinary Medicine, North Grafton, Massachusetts,1 Medarex, San Jose, California2

Received 9 April 2002/ Returned for modification 14 May 2002/ Accepted 24 June 2002

Hemolytic-uremic syndrome (HUS) is a serious complication which is predominantly associated in children with infection by Shiga toxin-producing Escherichia coli (STEC). By using HuMAb-Mouse (Medarex) animals, human monoclonal antibodies (Hu-MAbs) were developed against Shiga toxin 1 (Stx1) for passive immunotherapy of HUS. Ten stable hybridomas comprised of fully human heavy- and light-chain immunoglobulin elements and secreting Stx1-specific Hu-MAbs (seven immunoglobulin M({kappa}) [IgM({kappa})] elements [one specific for the A subunit and six specific for the B subunit] and three IgG1({kappa}) elements specific for subunit B) were isolated. Two IgM({kappa}) Hu-MAbs (2D9 and 15G9) and three IgG1({kappa}) Hu-MAbs (5A4, 10F4, and 15G2), all specific for subunit B, demonstrated marked neutralization of Stx1 in vitro and significant prolongation of survival in a murine model of Stx1 toxicosis.

* Corresponding author. Mailing address: Tufts University School of Veterinary Medicine, 200 Westboro Rd., Building 20, North Grafton, MA 01536. Phone: (508) 839-7955. Fax: (508) 839-7977. E-mail: Saul.Tzipori{at}tufts.edu.

Editor: J. D. Clements

Infection and Immunity, October 2002, p. 5896-5899, Vol. 70, No. 10
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.10.5896-5899.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Smith, M. J., Melton-Celsa, A. R., Sinclair, J. F., Carvalho, H. M., Robinson, C. M., O'Brien, A. D. (2009). Monoclonal Antibody 11E10, Which Neutralizes Shiga Toxin Type 2 (Stx2), Recognizes Three Regions on the Stx2 A Subunit, Blocks the Enzymatic Action of the Toxin In Vitro, and Alters the Overall Cellular Distribution of the Toxin. Infect. Immun. 77: 2730-2740 [Abstract] [Full Text]  
  • Kitov, P. I., Mulvey, G. L., Griener, T. P., Lipinski, T., Solomon, D., Paszkiewicz, E., Jacobson, J. M., Sadowska, J. M., Suzuki, M., Yamamura, K.-i., Armstrong, G. D., Bundle, D. R. (2008). From the Cover: In vivo supramolecular templating enhances the activity of multivalent ligands: A potential therapeutic against the Escherichia coli O157 AB5 toxins. Proc. Natl. Acad. Sci. USA 105: 16837-16842 [Abstract] [Full Text]  
  • Sauter, K. A. D., Melton-Celsa, A. R., Larkin, K., Troxell, M. L., O'Brien, A. D., Magun, B. E. (2008). Mouse Model of Hemolytic-Uremic Syndrome Caused by Endotoxin-Free Shiga Toxin 2 (Stx2) and Protection from Lethal Outcome by Anti-Stx2 Antibody. Infect. Immun. 76: 4469-4478 [Abstract] [Full Text]  
  • Krautz-Peterson, G., Chapman-Bonofiglio, S., Boisvert, K., Feng, H., Herman, I. M., Tzipori, S., Sheoran, A. S. (2008). Intracellular Neutralization of Shiga Toxin 2 by an A Subunit-Specific Human Monoclonal Antibody. Infect. Immun. 76: 1931-1939 [Abstract] [Full Text]  
  • Smith, M. J., Carvalho, H. M., Melton-Celsa, A. R., O'Brien, A. D. (2006). The 13C4 Monoclonal Antibody That Neutralizes Shiga Toxin Type 1 (Stx1) Recognizes Three Regions on the Stx1 B Subunit and Prevents Stx1 from Binding to Its Eukaryotic Receptor Globotriaosylceramide. Infect. Immun. 74: 6992-6998 [Abstract] [Full Text]  
  • Sheoran, A. S., Chapman-Bonofiglio, S., Harvey, B. R., Mukherjee, J., Georgiou, G., Donohue-Rolfe, A., Tzipori, S. (2005). Human Antibody against Shiga Toxin 2 Administered to Piglets after the Onset of Diarrhea Due to Escherichia coli O157:H7 Prevents Fatal Systemic Complications. Infect. Immun. 73: 4607-4613 [Abstract] [Full Text]  
  • Akiyoshi, D. E., Rich, C. M., O'Sullivan-Murphy, S., Richard, L., Dilo, J., Donohue-Rolfe, A., Sheoran, A. S., Chapman-Bonofiglio, S., Tzipori, S. (2005). Characterization of a Human Monoclonal Antibody against Shiga Toxin 2 Expressed in Chinese Hamster Ovary Cells. Infect. Immun. 73: 4054-4061 [Abstract] [Full Text]  
  • Tzipori, S., Sheoran, A., Akiyoshi, D., Donohue-Rolfe, A., Trachtman, H. (2004). Antibody Therapy in the Management of Shiga Toxin-Induced Hemolytic Uremic Syndrome. Clin. Microbiol. Rev. 17: 926-941 [Abstract] [Full Text]  
  • Sheoran, A. S., Chapman, S., Singh, P., Donohue-Rolfe, A., Tzipori, S. (2003). Stx2-Specific Human Monoclonal Antibodies Protect Mice against Lethal Infection with Escherichia coli Expressing Stx2 Variants. Infect. Immun. 71: 3125-3130 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»