This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Palaniappan, R. U. M.
Right arrow Articles by Mohammed, H. O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Palaniappan, R. U. M.
Right arrow Articles by Mohammed, H. O.

 Previous Article  |  Next Article 

Infection and Immunity, November 2002, p. 5924-5930, Vol. 70, No. 11
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.11.5924-5930.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Cloning and Molecular Characterization of an Immunogenic LigA Protein of Leptospira interrogans

Raghavan U. M. Palaniappan,1 Yung-Fu Chang,1* S. S. D. Jusuf,2 S. Artiushin,2 John F. Timoney,2 Sean P. McDonough,1 Steve C. Barr,1 Thomas J. Divers,1 Kenneth W. Simpson,1 Patrick L. McDonough,1 and Hussni O. Mohammed1

College of Veterinary Medicine, Cornell University, Ithaca, New York 14853,1 Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, Kentucky 405462

Received 16 January 2002/ Returned for modification 7 March 2002/ Accepted 24 July 2002

A clone expressing a novel immunoreactive leptospiral immunoglobulin-like protein A of 130 kDa (LigA) from Leptospira interrogans serovar pomona type kennewicki was isolated by screening a genomic DNA library with serum from a mare that had recently aborted due to leptospiral infection. LigA is encoded by an open reading frame of 3,675 bp, and the deduced amino acid sequence consists of a series of 90-amino-acid tandem repeats. A search of the NCBI database found that homology of the LigA repeat region was limited to an immunoglobulin-like domain of the bacterial intimin binding protein of Escherichia coli, the cell adhesion domain of Clostridium acetobutylicum, and the invasin of Yersinia pestis. Secondary structure prediction analysis indicates that LigA consists mostly of beta sheets with a few alpha-helical regions. No LigA was detectable by immunoblot analysis of lysates of the leptospires grown in vitro at 30°C or when cultures were shifted to 37°C. Strikingly, immunohistochemistry on kidney from leptospira-infected hamsters demonstrated LigA expression. These findings suggest that LigA is specifically induced only in vivo. Sera from horses, which aborted as a result of natural Leptospira infection, strongly recognize LigA. LigA is the first leptospiral protein described to have 12 tandem repeats and is also the first to be expressed only during infection. Thus, LigA may have value in serodiagnosis or as a protective immunogen in novel vaccines.

* Corresponding author. Mailing address: Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853. Phone: (607) 253-3675. Fax: (607) 253-3943. E-mail: yc42{at}cornell.edu.

Editor: D. L. Burns

Infection and Immunity, November 2002, p. 5924-5930, Vol. 70, No. 11
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.11.5924-5930.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Croda, J., Figueira, C. P., Wunder, E. A. Jr., Santos, C. S., Reis, M. G., Ko, A. I., Picardeau, M. (2008). Targeted Mutagenesis in Pathogenic Leptospira Species: Disruption of the LigB Gene Does Not Affect Virulence in Animal Models of Leptospirosis. Infect. Immun. 76: 5826-5833 [Abstract] [Full Text]  
  • Lin, Y.-P., Raman, R., Sharma, Y., Chang, Y.-F. (2008). Calcium Binds to Leptospiral Immunoglobulin-like Protein, LigB, and Modulates Fibronectin Binding. J. Biol. Chem. 283: 25140-25149 [Abstract] [Full Text]  
  • Matsunaga, J., Medeiros, M. A., Sanchez, Y., Werneid, K. F., Ko, A. I. (2007). Osmotic regulation of expression of two extracellular matrix-binding proteins and a haemolysin of Leptospira interrogans: differential effects on LigA and Sph2 extracellular release. Microbiology 153: 3390-3398 [Abstract] [Full Text]  
  • Matsunaga, J., Lo, M., Bulach, D. M., Zuerner, R. L., Adler, B., Haake, D. A. (2007). Response of Leptospira interrogans to Physiologic Osmolarity: Relevance in Signaling the Environment-to-Host Transition. Infect. Immun. 75: 2864-2874 [Abstract] [Full Text]  
  • Choy, H. A., Kelley, M. M., Chen, T. L., Moller, A. K., Matsunaga, J., Haake, D. A. (2007). Physiological Osmotic Induction of Leptospira interrogans Adhesion: LigA and LigB Bind Extracellular Matrix Proteins and Fibrinogen. Infect. Immun. 75: 2441-2450 [Abstract] [Full Text]  
  • Croda, J., Ramos, J. G. R., Matsunaga, J., Queiroz, A., Homma, A., Riley, L. W., Haake, D. A., Reis, M. G., Ko, A. I. (2007). Leptospira Immunoglobulin-Like Proteins as a Serodiagnostic Marker for Acute Leptospirosis. J. Clin. Microbiol. 45: 1528-1534 [Abstract] [Full Text]  
  • Barbosa, A. S., Abreu, P. A. E., Neves, F. O., Atzingen, M. V., Watanabe, M. M., Vieira, M. L., Morais, Z. M., Vasconcellos, S. A., Nascimento, A. L. T. O. (2006). A Newly Identified Leptospiral Adhesin Mediates Attachment to Laminin. Infect. Immun. 74: 6356-6364 [Abstract] [Full Text]  
  • Lo, M., Bulach, D. M., Powell, D. R., Haake, D. A., Matsunaga, J., Paustian, M. L., Zuerner, R. L., Adler, B. (2006). Effects of Temperature on Gene Expression Patterns in Leptospira interrogans Serovar Lai as Assessed by Whole-Genome Microarrays.. Infect. Immun. 74: 5848-5859 [Abstract] [Full Text]  
  • Palaniappan, R. U. M., McDonough, S. P., Divers, T. J., Chen, C.-S., Pan, M.-J., Matsumoto, M., Chang, Y.-F. (2006). Immunoprotection of Recombinant Leptospiral Immunoglobulin-Like Protein A against Leptospira interrogans Serovar Pomona Infection. Infect. Immun. 74: 1745-1750 [Abstract] [Full Text]  
  • Verma, A., Artiushin, S., Matsunaga, J., Haake, D. A., Timoney, J. F. (2005). LruA and LruB, Novel Lipoproteins of Pathogenic Leptospira interrogans Associated with Equine Recurrent Uveitis. Infect. Immun. 73: 7259-7266 [Abstract] [Full Text]  
  • Matsunaga, J., Sanchez, Y., Xu, X., Haake, D. A. (2005). Osmolarity, a Key Environmental Signal Controlling Expression of Leptospiral Proteins LigA and LigB and the Extracellular Release of LigA. Infect. Immun. 73: 70-78 [Abstract] [Full Text]  
  • Palaniappan, R. U.M., Chang, Y.-F., Hassan, F., McDonough, S. P., Pough, M., Barr, S. C., Simpson, K. W., Mohammed, H. O., Shin, S., McDonough, P., Zuerner, R. L., Qu, J., Roe, B. (2004). Expression of leptospiral immunoglobulin-like protein by Leptospira interrogans and evaluation of its diagnostic potential in a kinetic ELISA. J Med Microbiol 53: 975-984 [Abstract] [Full Text]  
  • Nascimento, A. L. T. O., Ko, A. I., Martins, E. A. L., Monteiro-Vitorello, C. B., Ho, P. L., Haake, D. A., Verjovski-Almeida, S., Hartskeerl, R. A., Marques, M. V., Oliveira, M. C., Menck, C. F. M., Leite, L. C. C., Carrer, H., Coutinho, L. L., Degrave, W. M., Dellagostin, O. A., El-Dorry, H., Ferro, E. S., Ferro, M. I. T., Furlan, L. R., Gamberini, M., Giglioti, E. A., Goes-Neto, A., Goldman, G. H., Goldman, M. H. S., Harakava, R., Jeronimo, S. M. B, Junqueira-de-Azevedo, I. L. M., Kimura, E. T., Kuramae, E. E., Lemos, E. G. M., Lemos, M. V. F., Marino, C. L., Nunes, L. R., de Oliveira, R. C., Pereira, G. G., Reis, M. S., Schriefer, A., Siqueira, W. J., Sommer, P., Tsai, S. M., Simpson, A. J. G., Ferro, J. A., Camargo, L. E. A., Kitajima, J. P., Setubal, J. C., Van Sluys, M. A. (2004). Comparative Genomics of Two Leptospira interrogans Serovars Reveals Novel Insights into Physiology and Pathogenesis. J. Bacteriol. 186: 2164-2172 [Abstract] [Full Text]  
  • Artiushin, S., Timoney, J. F., Nally, J., Verma, A. (2004). Host-Inducible Immunogenic Sphingomyelinase-Like Protein, Lk73.5, of Leptospira interrogans. Infect. Immun. 72: 742-749 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»