This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Raghavan, S.
Right arrow Articles by Svennerholm, A.-M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Raghavan, S.
Right arrow Articles by Svennerholm, A.-M.

 Previous Article  |  Next Article 

Infection and Immunity, November 2002, p. 6383-6388, Vol. 70, No. 11
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.11.6383-6388.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Protection against Experimental Helicobacter pylori Infection after Immunization with Inactivated H. pylori Whole-Cell Vaccines

S. Raghavan, M. Hjulström, J. Holmgren, and A.-M. Svennerholm*

Department of Medical Microbiology and Immunology and Göteborg University Vaccine Research Institute (GUVAX), Göteborg University, S 41346 Göteborg, Sweden

Received 19 December 2001/ Returned for modification 26 February 2002/ Accepted 25 July 2002

The protective effect of therapeutic oral immunization with homologous and heterologous formalin-inactivated Helicobacter pylori cells given together with cholera toxin as an adjuvant was evaluated with C57BL/6 mice infected with H. pylori Sydney strain 1 (SS1). The bacteria used for immunization were strains that were either homologous or heterologous with regard to the O antigen (i.e., the Lewis antigen [Le antigen]) expressed by the lipopolysaccharide of the infecting H. pylori SS1 strain. We found that repeated oral immunization with inactivated H. pylori SS1 cells can significantly inhibit an existing infection (P < 0.001) and that the protection induced by such therapeutic immunization extends to protection against reinfection (P < 0.001). A similar level of protection was also achieved by immunization with another inactivated H. pylori strain having the same O antigen (Le antigen) as the infecting H. pylori SS1 strain. In contrast, immunization with inactivated strains expressing a heterologous O antigen, Lex, provided less protection or no protection. Immunization with H. pylori lysate preparations, on the other hand, resulted in significant comparable protection whether the lysates were prepared from an Lex strain or an Ley strain. Postimmunization gastritis was seen in mice that were protected after vaccination but not in unimmunized or unprotected mice. In conclusion, therapeutic immunization with inactivated H. pylori whole-cell vaccines may provide strong protection both against experimental H. pylori infection and against later reinfection.

* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, Guldhedsgatan 10A, S-413 46 Göteborg, Sweden. Phone: 46 31 342 4724. Fax: 46 31 826976. E-mail: ann-mari.svennerholm{at}microbio.gu.se.

Editor: S. H. E. Kaufmann

Infection and Immunity, November 2002, p. 6383-6388, Vol. 70, No. 11
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.11.6383-6388.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gorrell, R. J., Robins-Browne, R. M. (2009). Antibody-Mediated Protection against Infection with Helicobacter pylori in a Suckling Mouse Model of Passive Immunity. Infect. Immun. 77: 5116-5129 [Abstract] [Full Text]  
  • Chionh, Y.-T., Wee, J. L. K., Every, A. L., Ng, G. Z., Sutton, P. (2009). M-Cell Targeting of Whole Killed Bacteria Induces Protective Immunity against Gastrointestinal Pathogens. Infect. Immun. 77: 2962-2970 [Abstract] [Full Text]  
  • Hifumi, E., Morihara, F., Hatiuchi, K., Okuda, T., Nishizono, A., Uda, T. (2008). Catalytic Features and Eradication Ability of Antibody Light-chain UA15-L against Helicobacter pylori. J. Biol. Chem. 283: 899-907 [Abstract] [Full Text]  
  • Morihara, F., Fujii, R., Hifumi, E., Nishizono, A., Uda, T. (2007). Effects of vaccination by a recombinant antigen ureB138 (a segment of the {beta}-subunit of urease) against Helicobacter pylori infection. J Med Microbiol 56: 847-853 [Abstract] [Full Text]  
  • Carlsohn, E., Nystrom, J., Bolin, I., Nilsson, C. L., Svennerholm, A.-M. (2006). HpaA Is Essential for Helicobacter pylori Colonization in Mice. Infect. Immun. 74: 920-926 [Abstract] [Full Text]  
  • Yun, C. H., Lundgren, A., Azem, J., Sjoling, A., Holmgren, J., Svennerholm, A.-M., Lundin, B. S. (2005). Natural Killer Cells and Helicobacter pylori Infection: Bacterial Antigens and Interleukin-12 Act Synergistically To Induce Gamma Interferon Production. Infect. Immun. 73: 1482-1490 [Abstract] [Full Text]  
  • Salaun, L., Ayraud, S., Saunders, N. J. (2005). Phase variation mediated niche adaptation during prolonged experimental murine infection with Helicobacter pylori. Microbiology 151: 917-923 [Abstract] [Full Text]  
  • Sommer, F., Wilken, H., Faller, G., Lohoff, M. (2004). Systemic Th1 Immunization of Mice against Helicobacter pylori Infection with CpG Oligodeoxynucleotides as Adjuvants Does Not Protect from Infection but Enhances Gastritis. Infect. Immun. 72: 1029-1035 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»