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Infection and Immunity, November 2002, p. 6444-6447, Vol. 70, No. 11
0019-9567/02/$04.00+0 DOI: 10.1128/IAI.70.11.6444-6447.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.
Selection of Mutant Cell Lines Resistant to Infection by Chlamydia trachomatis and Chlamydia pneumoniae
Trevor Fudyk,1 Lynn Olinger,2 and Richard S. Stephens1,2*
Division of Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720,1
Francis I. Proctor Foundation, University of California, San Francisco, California 941432
Received 11 June 2002/
Returned for modification 23 July 2002/
Accepted 31 July 2002
The lytic outcome of natural infection by Chlamydia trachomatis was exploited to select CHO (Chinese hamster ovary) cells, following chemical mutagenesis, that were deficient in their ability to sustain productive chlamydial infection. Four distinct mutant cell phenotypes with defects in either attachment or postattachment mechanisms that are required for infection by C. trachomatis and Chlamydia pneumoniae were characterized.
* Corresponding author. Mailing address: Division of Infectious Diseases, School of Public Health, 140 Earl Warren Hall, University of California, Berkeley, CA 94720-7360. Phone: (510) 643-9900. Fax: (510) 643-1537. E-mail: rss{at}uclink4.berkeley.edu.
Editor: D. L. Burns
Infection and Immunity, November 2002, p. 6444-6447, Vol. 70, No. 11
0019-9567/02/$04.00+0 DOI: 10.1128/IAI.70.11.6444-6447.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.
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Copyright © 2002 by the American Society for Microbiology. All rights reserved.