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Infection and Immunity, November 2002, p. 6468-6470, Vol. 70, No. 11
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.11.6468-6470.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Characterization and Immunogenicity of the CagF Protein of the cag Pathogenicity Island of Helicobacter pylori

Anke Seydel,1,2 Elisabetta Tasca,1,2 Duccio Berti,3 Rino Rappuoli,3 Giuseppe Del Giudice,3 and Cesare Montecucco1,2*

Dipartimento di Scienze Biomediche Sperimentali, Università degli Studi di Padova,1 Istituto Veneto di Medicina Molecolare, I-35121 Padua,2 Centro di Ricerche IRIS, Chiron S.p.A., I-53100 Siena, Italy3

Received 6 June 2002/ Returned for modification 15 July 2002/ Accepted 5 August 2002

Helicobacter pylori infection causes severe gastroduodenal diseases in humans. Its virulence is strongly increased by the presence of the cag pathogenicity island (cag PAI). It has been shown that CagA, a major antigen in humans, is translocated to the host cell via a secretion system encoded by the cag PAI. The roles of many of the proteins encoded within the cag PAI are not known. Here we report on the cloning and expression of CagF, one of those proteins. We show that CagF is associated to the outer membrane of H. pylori G27 and that the protein is always expressed with electrophoretic mobility variations among the 20 strains tested here. We have also found that natural infection with H. pylori is able to induce antibodies against CagF.

* Corresponding author. Mailing address: Università degli Studi di Padova, Dipartimento di Scienze Biomediche Sperimentali, viale G. Colombo 3, I-35121 Padua, Italy. Phone: 39 049 827 6058. Fax: 39 049 827 6049. E-mail: cesare.montecucco{at}unipd.it.

Editor: J. T. Barbieri

Infection and Immunity, November 2002, p. 6468-6470, Vol. 70, No. 11
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.11.6468-6470.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pattis, I., Weiss, E., Laugks, R., Haas, R., Fischer, W. (2007). The Helicobacter pylori CagF protein is a type IV secretion chaperone-like molecule that binds close to the C-terminal secretion signal of the CagA effector protein. Microbiology 153: 2896-2909 [Abstract] [Full Text]  
  • Couturier, M. R., Tasca, E., Montecucco, C., Stein, M. (2006). Interaction with CagF Is Required for Translocation of CagA into the Host via the Helicobacter pylori Type IV Secretion System. Infect. Immun. 74: 273-281 [Abstract] [Full Text]  
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