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Infection and Immunity, November 2002, p. 6475-6480, Vol. 70, No. 11
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.11.6475-6480.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Comparative Proteomic Analysis of Extracellular Proteins of Edwardsiella tarda

Department of Biological Sciences, Faculty of Science,¹ Tropical Marine Science Institute, the National University of Singapore, Singapore 117543²

Received 8 April 2002/ Returned for modification 18 June 2002/ Accepted 31 July 2002

A comparison of extracellular proteins of virulent and avirulent Edwardsiella tarda strains revealed several major, virulent-strain-specific proteins. Proteomic analysis identified two of the proteins in the virulent strain PPD130/91 as flagellin and SseB, which are virulence factors in bacterial pathogens. PCR amplification and DNA sequencing confirmed the presence of the genes that encode these proteins. Our results clearly demonstrated the potency of the proteomic approach in identifying virulence factors.

Editor: B. B. Finlay

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