This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Purkall, D.
Right arrow Articles by Schenkein, H. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Purkall, D.
Right arrow Articles by Schenkein, H. A.

 Previous Article  |  Next Article 

Infection and Immunity, November 2002, p. 6485-6488, Vol. 70, No. 11
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.11.6485-6488.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Opsonization of Actinobacillus actinomycetemcomitans by Immunoglobulin G Antibody Reactive with Phosphorylcholine

Donald Purkall, John G. Tew, and Harvey A. Schenkein*

Clinical Research Center for Periodontal Disease, School of Dentistry, Virginia Commonwealth University, Richmond, Virginia 23298

Received 9 May 2002/ Returned for modification 2 July 2002/ Accepted 5 August 2002

We used two strains of Actinobacillus actinomycetemcomitans, one bearing phosphorylcholine (PC) (strain D045D-40) and one devoid of PC antigens (strain DB03A-42), as well as a nonencapsulated strain of Streptococcus pneumoniae (strain 39937), to examine the opsonic properties of physiological concentrations (<=30 µg/ml) of purified human anti-PC immunoglobulin G (IgG). Anti-PC bound to both A. actinomycetemcomitans DO45D-40 and S. pneumoniae 39937 and induced superoxide anion production by polymorphonuclear neutrophils; induction of the oxidative burst was inhibited by antibodies to either CD16 or CD32. Thus, anti-PC IgG at concentrations present in most human sera promotes the opsonization of PC-expressing strains of A. actinomycetemcomitans in the absence of complement, implying that anti-PC may be a protective antibody against such strains of bacteria.

* Corresponding author. Mailing address: School of Dentistry, Virginia Commonwealth University, P.O. Box 980566, Richmond, VA 23298. Phone: (804) 828-9185. Fax: (804) 828-5787. E-mail: haschenk{at}vcu.edu.

Editor: J. D. Clements

Infection and Immunity, November 2002, p. 6485-6488, Vol. 70, No. 11
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.11.6485-6488.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»