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Infection and Immunity, December 2002, p. 6652-6657, Vol. 70, No. 12
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.12.6652-6657.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Marked Enhancement of the Antigen-Specific Immune Response by Combining Plasmid DNA-Based Immunization with a Schiff Base-Forming Drug

Jehad Charo,1,2* Maria Sundbäck,1 Ken Wasserman,3 Anne-Marie T. Ciupitu,1 Babak Mirzai,2 Ruurd van der Zee,4 and Rolf Kiessling1,2

Cancer Center Karolinska (CCK), Karolinska Hospital, S-17176,1 Microbiology and Tumor Biology Center (MTC), Karolinska Institute, S-17177, Stockholm, Sweden,2 American Type Culture Collection, Manassas, Virginia 20110,3 Department of Immunology, Faculty of Veterinary Medicine, Utrecht University, 3508 TD Utrecht, The Netherlands4

Received 20 May 2002/ Returned for modification 28 June 2002/ Accepted 13 September 2002

Although plasmid DNA (pDNA)-based immunization has proven efficacy, the level of immune responses that is achieved by this route of vaccination is often lower than that induced by traditional vaccines, especially for primates and humans. We report here a simple and potent method to enhance pDNA-based vaccination by using two different plasmids encoding viral or bacterial antigens. This method is based on coadministration of low concentrations of a recently described immunopotentiating, Schiff base-forming drug called tucaresol which has led to significant augmentation of antigen-specific humoral and cellular immune responses. Our data suggest that enhancement of the immune response with tucaresol might provide a powerful tool for the further development of pDNA-based immunization for humans.

* Corresponding author. Present address: Surgery Branch, National Cancer Institute, National Institutes of Health, Building 10, Room 2B42, 10 Center Dr., Bethesda, MD 20892. Phone: (301) 496-9383. Fax: (301) 496-0011. E-mail: Jehad_Charo{at}nih.gov.

Editor: J. D. Clements

Infection and Immunity, December 2002, p. 6652-6657, Vol. 70, No. 12
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.12.6652-6657.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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