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Infection and Immunity, December 2002, p. 6680-6687, Vol. 70, No. 12
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.12.6680-6687.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Analysis of Ebh, a 1.1-Megadalton Cell Wall-Associated Fibronectin-Binding Protein of Staphylococcus aureus

Simon R. Clarke,1 Llinos G. Harris,1,2 R. Geoff Richards,2 and Simon J. Foster1*

Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom,1 AO Research Institute, CH7270 Davos, Switzerland2

Received 14 June 2002/ Returned for modification 29 July 2002/ Accepted 27 August 2002

In order for Staphylococcus aureus to adhere to host extracellular matrix (ECM) substrates, it elicits a wide range of surface proteins. We have characterized a novel ~1.1-MDa protein in S. aureus, termed Ebh (for ECM-binding protein homologue), which has homology to other ECM-binding proteins. Ebh consists of several domains, including a large central region with 44 imperfect repeats of 126 amino acids. Expression analysis revealed ebh to be growth phase regulated and repressed by agr. A fragment of the central repeat region of Ebh was cloned, overexpressed, and used in ligand-binding studies to determine Ebh function. The recombinant protein was found to specifically bind human fibronectin. Ebh is produced during human infection since serum samples taken from patients with confirmed S. aureus infections were found to contain anti-Ebh antibodies. Localization studies revealed Ebh to be cell envelope associated and is proposed to form a specialized surface structure involved in cellular adhesion.

* Corresponding author. Mailing address: Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, United Kingdom. Phone: 44-114-222-4411. Fax: 44-114-272-8697. E-mail: s.foster{at}sheffield.ac.uk.

Editor: B. B. Finlay

Infection and Immunity, December 2002, p. 6680-6687, Vol. 70, No. 12
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.12.6680-6687.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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