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Infection and Immunity, December 2002, p. 6805-6810, Vol. 70, No. 12
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.12.6805-6810.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Identification of a Fibronectin-Binding Protein from Staphylococcus epidermidis

Rachel J. Williams, Brian Henderson, Lindsay J. Sharp, and Sean P. Nair*

Cellular Microbiology Research Group, Eastman Dental Institute for Oral Health Care Sciences, University College London, London WC1X 8LD, United Kingdom

Received 5 June 2002/ Returned for modification 12 July 2002/ Accepted 23 August 2002

Staphylococcus epidermidis has been reported to bind to a number of host cell extracellular matrix proteins, including fibronectin. Here we report the identification of a fibronectin-binding protein from S. epidermidis. A phage display library of S. epidermidis genomic DNA was constructed and panned against immobilized fibronectin. A number of phagemid clones containing overlapping inserts were identified, and one of these clones, pSE109FN, contained a 1.4-kb insert. Phage pSE109FN was found to bind to fibronectin but not to collagen, fibrinogen, laminin, or vitronectin. However, pSE109FN also bound to heparin, hyaluronate, and plasminogen, although to a lesser extent than it bound to fibronectin. Analysis of The Institute for Genomic Research S. epidermidis genome sequence database revealed a 1.85-kb region within a putative 30.5-kb open reading frame, to which the overlapping DNA inserts contained within the fibronectin-binding phagemids mapped. We have designated the gene encoding the fibronectin-binding domain embp. A recombinant protein, Embp32, which encompassed the fibronectin-binding domain of Embp, blocked the binding of S. epidermidis, but not the binding of Staphylococcus aureus, to fibronectin. In contrast, a recombinant protein, FnBPB[D1-D4], spanning the fibronectin-binding domain of the S. aureus fibronectin-binding protein FnBPB, blocked binding of S. aureus to fibronectin but had a negligible effect on the binding of S. epidermidis.

* Corresponding author. Mailing address: Cellular Microbiology Research Group, Eastman Dental Institute, University College London, 256 Gray's Inn Road, London WC1X 8LD, United Kingdom. Phone: 44 (0)20 7915 1118. Fax: 44 (0)20 7915 1127. E-mail: snair{at}eastman.ucl.ac.uk.

Editor: D. L. Burns

Infection and Immunity, December 2002, p. 6805-6810, Vol. 70, No. 12
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.12.6805-6810.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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