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Infection and Immunity, December 2002, p. 6987-6995, Vol. 70, No. 12
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.12.6987-6995.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Cryptosporidium parvum Genes Containing Thrombospondin Type 1 Domains

Mingqi Deng,1 Thomas J. Templeton,2 Nicole R. London,1 Carrey Bauer,1 Alison A. Schroeder,1 and Mitchell S. Abrahamsen1*

Department of Veterinary PathoBiology, University of Minnesota, St. Paul, Minnesota 55108,1 Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, New York 100212

Received 8 March 2002/ Returned for modification 23 May 2002/ Accepted 23 August 2002

Cryptosporidium parvum is recognized as an enteropathogen of great worldwide medical and veterinary importance, yet understanding of its pathogenesis has been hampered in part by limited knowledge of the invasion machinery of this parasite. Recently, genes containing thrombospondin type 1 (TSP1) domains have been identified in several genera of apicomplexans, including thrombospondin-related adhesive proteins (TRAPs) that have been implicated as key molecules for parasite motility and adhesion onto host cell surfaces. Previously, a large-scale random survey of the C. parvum genome conducted in our laboratory revealed the presence of multiple genomic DNA sequences with a high degree of similarity to known apicomplexan TRAP genes. In the present study, TBLASTN screening of available C. parvum genomic sequences by using TSP1 domains as queries identified a total of 12 genes possessing TSP1-like domains. All genes have putative signal peptide sequences, one or more TSP1-like domains, plus additional extracellular protein modules such as Kringle, epidermal growth factor, and Apple domains. Two genes, putative paralogs CpTSP8 and CpTSP9, contain predicted introns near their amino termini, which were verified by comparing PCR products from cDNA versus genomic DNA templates. Reverse transcription-PCR analysis of transcript levels reveals that C. parvum TSP genes were developmentally regulated with distinct patterns of expression during in vitro infection. TRAPC1, CpTSP3, and CpTSP11 were expressed at high levels during both early and late stages of infection, whereas CpTSP2, CpTSP5, CpTSP6, CpTSP8, and CpTSP9 were maximally expressed during the late stages of infection. Only CpTSP4 was highly expressed solely at an early stage of infection.

* Corresponding author. Mailing address: Department of Veterinary PathoBiology, University of Minnesota, St. Paul, MN 55108. Phone: (612) 624-1244. Fax: (612) 625-0204. E-mail: abe{at}umn.edu.

Editor: J. M. Mansfield

Infection and Immunity, December 2002, p. 6987-6995, Vol. 70, No. 12
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.12.6987-6995.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Cai, X., Herschap, D., Zhu, G. (2005). Functional Characterization of an Evolutionarily Distinct Phosphopantetheinyl Transferase in the Apicomplexan Cryptosporidium parvum. Eukaryot Cell 4: 1211-1220 [Abstract] [Full Text]  
  • Cai, X., Lancto, C. A., Abrahamsen, M. S., Zhu, G. (2004). Intron-containing {beta}-tubulin transcripts in Cryptosporidium parvum cultured in vitro. Microbiology 150: 1191-1195 [Abstract] [Full Text]  
  • Templeton, T. J., Lancto, C. A., Vigdorovich, V., Liu, C., London, N. R., Hadsall, K. Z., Abrahamsen, M. S. (2004). The Cryptosporidium Oocyst Wall Protein Is a Member of a Multigene Family and Has a Homolog in Toxoplasma. Infect. Immun. 72: 980-987 [Abstract] [Full Text]  


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