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Infection and Immunity, December 2002, p. 7136-7139, Vol. 70, No. 12
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.12.7136-7139.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

The Furin Inhibitor Hexa-D-Arginine Blocks the Activation of Pseudomonas aeruginosa Exotoxin A In Vivo

Miroslav S. Sarac, Angus Cameron,{dagger} and Iris Lindberg*

Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70112

Received 30 May 2002/ Returned for modification 6 August 2002/ Accepted 20 August 2002

The Pseudomonas aeruginosa exotoxin A (PEA) protein requires furin-mediated cleavage for manifestation of toxicity. We show here that the small stable furin inhibitor hexa-D-arginine amide effectively blocks PEA-induced cell lysis and is itself noncytotoxic. Administration of hexa-D-arginine to PEA-treated mice significantly improves their survival rate and also decreases circulating levels of tumor necrosis factor alpha.

* Corresponding author. Mailing address: Louisiana State University Health Sciences Center, Department of Biochemistry and Molecular Biology, 1901 Perdido St., New Orleans, LA 70112. Phone: (504) 568-4799. Fax: (504) 568-6598. E-mail: ilindb{at}lsuhsc.edu.

Editor: J. T. Barbieri

{dagger} Present address: Biochemistry Department, School of Medical Sciences, University of Bristol, Bristol, United Kingdom BS8 1TD.

Infection and Immunity, December 2002, p. 7136-7139, Vol. 70, No. 12
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.12.7136-7139.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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