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Infection and Immunity, December 2002, p. 7140-7144, Vol. 70, No. 12
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.12.7140-7144.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Gene Discovery in the Freshwater Fish Parasite Trypanosoma carassii: Identification of trans-Sialidase-Like and Mucin-Like Genes

Fernán Agüero,1 Vanina Campo,1 Laura Cremona,1,{dagger} Adriana Jäger,1 Javier M. Di Noia,1,{ddagger} Peter Overath,2 Daniel O. Sánchez,1* and Alberto Carlos Frasch1*

Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús, Universidad Nacional de General San Martín-CONICET, San Martín, 1650 Buenos Aires, Argentina,1 Max-Planck-Institut fur Biologie, Abteilung Membranbiochemie, D-72076, Tübingen, Germany2

Received 30 May 2002/ Returned for modification 27 July 2002/ Accepted 8 September 2002

A total of 1,921 expressed sequence tags (ESTs) were obtained from bloodstream trypomastigotes of Trypanosoma carassii, a parasite of economic importance due to its high prevalence in fish farms. Analysis of the data set allowed us to identify a trans-sialidase (TS)-like gene and three ESTs coding for putative mucin-like genes. TS activity was detected in cell extracts of bloodstream trypomastigotes. We have also used the sequence information obtained to identify genes that have not been previously described in trypanosomatids. (Additional information on these ESTs can be found at http://genoma.unsam.edu.ar/projects/tca.)

* Corresponding authors. Mailing address: Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús, Universidad Nacional de General San Martín-CONICET, San Martín, 1650, Buenos Aires, Argentina. Phone: 54-11-4580-7255. Fax: 54-11-4752-9639. E-mail address for D. O. Sánchez: dsanchez{at}iib.unsam.edu.ar. E-mail address for A. C. Frasch: cfrasch{at}iib.unsam.edu.ar.

Editor: W. A. Petri, Jr.

{dagger} Present address: Cellular Biochemistry and Biophysics, Memorial Sloan-Kettering Cancer Center, New York, NY 10021.

{ddagger} Present address: MRC Laboratory of Molecular Biology, PNAC Unit, Cambridge CB2 2QH, United Kingdom.

Infection and Immunity, December 2002, p. 7140-7144, Vol. 70, No. 12
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.12.7140-7144.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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