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Infection and Immunity, December 2002, p. 7156-7160, Vol. 70, No. 12
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.12.7156-7160.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

IroN Functions as a Siderophore Receptor and Is a Urovirulence Factor in an Extraintestinal Pathogenic Isolate of Escherichia coli

Thomas A. Russo,1,2,3,4* Catherine D. McFadden,1,3 Ulrike B. Carlino-MacDonald,1,3 Janet M. Beanan,1,3 Travis J. Barnard,5 and James R. Johnson6

Department of Medicine,1 Department of Microbiology,2 The Witebsky Center for Microbial Pathogenesis,3 VA Medical Center, University of Buffalo, Buffalo, New York 14214,4 Department of Molecular Microbiology and Immunology, University of Missouri School of Medicine, Columbia, Missouri,5 Medical Service, VA Medical Center, and Department of Medicine, University of Minnesota, Minneapolis, Minnesota6

Received 14 May 2002/ Returned for modification 19 August 2002/ Accepted 4 September 2002

IroN was recently identified in the extracellular pathogenic Escherichia coli strain CP9. In this study experimental evidence demonstrating that IroN mediates utilization of the siderophore enterobactin was obtained, thereby establishing IroN as a catecholate siderophore receptor. In a mouse model of ascending urinary tract infection the presence of iroN contributed significantly to CP9's ability to colonize the mouse bladder, kidneys, and urine, evidence that IroN is a urovirulence factor. However, growth in human urine ex vivo and adherence to uroepithelial cells in vitro were equivalent for an isogenic mutant deficient in IroN (CP82) and its wild-type parent (CP9). Taken together, these findings establish that IroN is a siderophore receptor and a urovirulence factor. However, uncertainty exists as to the mechanism(s) via which IroN contributes to urovirulence.

* Corresponding author. Mailing address: Department of Medicine, Division of Infectious Diseases, 3435 Main St., Biomedical Research Building, Room 141, Buffalo, NY 14214. Phone: (716) 829-2674. Fax: (716) 829-3889. E-mail: trusso{at}acsu.buffalo.edu.

Editor: B. B. Finlay

Infection and Immunity, December 2002, p. 7156-7160, Vol. 70, No. 12
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.12.7156-7160.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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