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Infection and Immunity, December 2002, p. 7165-7168, Vol. 70, No. 12
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.12.7165-7168.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Rough Lipopolysaccharide from Brucella abortus and Escherichia coli Differentially Activates the Same Mitogen-Activated Protein Kinase Signaling Pathways for Tumor Necrosis Factor Alpha in RAW 264.7 Macrophage-Like Cells

Bruce W. Jarvis, Tajie H. Harris, Nilofer Qureshi, and Gary A. Splitter^*

Animal Health and Biomedical Sciences Department, University of Wisconsin, Madison, Wisconsin 53706

Received 10 April 2002/ Returned for modification 29 June 2002/ Accepted 20 August 2002

The intracellular, gram-negative pathogen Brucella abortus establishes chronic infections in host macrophages while downregulating cytokines such as tumor necrosis factor alpha (TNF-). When producing TNF-, Brucella abortus rough lipopolysaccharide (LPS) activates the same mitogen-activated protein kinase signaling pathways (ERK and JNK) as Escherichia coli LPS, but Brucella LPS is a much less potent agonist.

Editor: V. J. DiRita

Present address: Department of Basic Medical Sciences, Medical School, University of Missouri, Kansas City, MO 64108.

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