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Infection and Immunity, December 2002, p. 7172-7175, Vol. 70, No. 12
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.12.7172-7175.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Leptospires Are Killed In Vitro by Both Oxygen-Dependent and -Independent Reactions

Rossella Murgia,1 Rodolfo Garcia,2 and Marina Cinco1*

Department of Biomedical Sciences, University of Trieste, 34127 Trieste,1 International Centre for Genetic Engineering and Biotechnology, 34012 Trieste, Italy2

Received 27 June 2002/ Returned for modification 7 August 2002/ Accepted 11 September 2002

This study reports for the first time that leptospires are killed by H2O2 and by low-molecular-weight primary granule components, which are agents normally released by neutrophils upon stimulation. Although both pathogenic and nonpathogenic strains were sensitive to H2O2-mediated killing, nonpathogenic organisms were found to be more susceptible. In addition, the killing of leptospires by H2O2 was found to be independent of the presence of the neutrophil primary granule component myeloperoxidase and therefore not a consequence of halogenation reactions. We have also determined that leptospires are significantly sensitive only to primary granule components and, among those, to proteins and/or peptides of less than 30 kDa.

* Corresponding author. Mailing address: Department of Biomedical Sciences, University of Trieste, 34127 Trieste, Italy. Phone: 39-040-5587178. Fax: 39-040-351668. E-mail: cinco{at}dsbmail.units.it.

Editor: B. B. Finlay

Infection and Immunity, December 2002, p. 7172-7175, Vol. 70, No. 12
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.12.7172-7175.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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