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Infection and Immunity, February 2002, p. 1010-1013, Vol. 70, No. 2
0019-9567/01/$04.00+0     DOI: 70.2.1010-1013.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Antigenic Specificity of the Mycobacterium leprae Homologue of ESAT-6

John S. Spencer,1* Maria Angela M. Marques,1 Monica C. B. S. Lima,1,2,3 Ana Paula Junqueira-Kipnis,1 Bruce C. Gregory,1 Richard W. Truman,4 and Patrick J. Brennan1

Department of Microbiology, Colorado State University, Fort Collins, Colorado; Hansen’s Disease Laboratory, Oswaldo Cruz Institute, Oswaldo Cruz Foundation,,1 and Laboratory of Immunopathology, School of Medical Sciences, State University of Rio de Janeiro, Rio de Janeiro, Brazil;,2 National Hansen's Disease Center, Louisiana State University, Baton Rouge, Louisiana3

Received 9 August 2001/ Returned for modification 1 October 2001/ Accepted 31 October 2001

The sequence of the Mycobacterium leprae homologue of ESAT-6 shows only 36% amino acid correspondence to that from Mycobacterium tuberculosis. Anti-M. leprae ESAT-6 polyclonal and monoclonal antibodies and T-cell hybridomas reacted only with the homologous protein and allowed identification of the B- and T-cell epitopes. The protein is expressed in M. leprae and appears in the cell wall fraction. Thus, M. leprae ESAT-6 shows promise as a specific diagnostic agent for leprosy.

* Corresponding author. Mailing address: Department of Microbiology, Colorado State University, Fort Collins, CO 80523-1677. Phone: (970) 491-2432. Fax: (970) 491-1815. E-mail: john.spencer{at}colostate.edu.

Editor: J. M. Mansfield

Infection and Immunity, February 2002, p. 1010-1013, Vol. 70, No. 2
0019-9567/01/$04.00+0     DOI: 70.2.1010-1013.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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