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Infection and Immunity, February 2002, p. 1014-1016, Vol. 70, No. 2
0019-9567/01/$04.00+0     DOI: 70.2.1014-1016.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Conformationally Correct Expression of Membrane-Anchored Toxoplasma gondii SAG1 in the Primitive Protozoan Giardia duodenalis

Matthias Marti, Yajie Li,,{dagger} Peter Köhler, and Adrian B. Hehl*

Institute of Parasitology, University of Zürich, CH-8057 Zürich, Switzerland

Received 13 August 2001/ Returned for modification 19 September 2001/ Accepted 12 November 2001

To explore the possibility of expressing membrane-anchored exodomains of heterologous surface antigens in Giardia, a chimeric construct containing the Toxoplasma gondii SAG1 gene was made. The Giardia system is shown here to provide a means of generating correctly folded chimeric surface proteins in a native and unmodified form.

* Corresponding author. Mailing address: Institute of Parasitology, University of Zürich, Winterthurerstrasse 266a, CH-8057 Zürich, Switzerland, Phone: 41-1-635-8526. Fax: 41-1-635-8907. E-mail: ahehl{at}vetparas.unizh.ch.

Editor: J. M. Mansfield

{dagger} Present address: Department of Parasitology, Harbin Medical University, 150086 Harbin, People's Republic of China

Infection and Immunity, February 2002, p. 1014-1016, Vol. 70, No. 2
0019-9567/01/$04.00+0     DOI: 70.2.1014-1016.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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