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Infection and Immunity, February 2002, p. 544-560, Vol. 70, No. 2
0019-9567/01/$04.00+0     DOI: 10.1128/IAI.70.2.544-550.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Efficiency of Protection of Guinea Pigs against Infection with Bacillus anthracis Spores by Passive Immunization

David Kobiler,1 Yehoshua Gozes,1 Hagai Rosenberg,2 Dino Marcus,3 Shaul Reuveny,3 and Zeev Altboum1*

Departments of Infectious Diseases,1 Biochemistry and Molecular Genetics,2 Biotechnology, Israel Institute for Biological Research, Ness-Ziona 74100, Israel3

Received 2 July 2001/ Accepted 5 November 2001

The efficacy of passive immunization as a postexposure prophylactic measure for treatment of guinea pigs intranasally infected with Bacillus anthracis spores was evaluated. Antisera directed either against the lethal toxin components (PA or LF) or against a toxinogenic strain (Sterne) were used for this evaluation. All antisera exhibited high enzyme-linked immunosorbent assay titers against the corresponding antigens, high titers of neutralization of cytotoxicity activity in an in vitro mouse macrophages cell line (J774A.1), as well as in vivo neutralization of toxicity when administered either directly to Fisher rats prior to challenge with the lethal toxin or after incubation with the lethal toxin. In these tests, anti-LF antiserum exhibited the highest neutralization efficiency, followed by anti-Sterne and anti-PA. The time dependence and antibody dose necessary for conferring postexposure protection by the various antibodies of guinea pigs infected with 25 50% lethal doses of Vollum spores was examined. Rabbit anti-PA serum was found to be the most effective. Intraperitoneal injections of anti-PA serum given 24 h postinfection protected 90% of the infected animals, whereas anti-Sterne and anti-LF were less effective. These results further emphasizes the importance of anti-PA antibodies in conferring protection against B. anthracis infection and demonstrated the ability of such antibodies to be effectively applied as an efficient postexposure treatment against anthrax disease.

* Corresponding author. Mailing address: Department of Infectious Diseases, Israel Institute for Biological Research, P.O. Box 19, Ness-Ziona 74100, Israel. Phone: 972-8-9381414. Fax: 972-8-9381639. E-mail: altboum{at}iibr.gov.il.

Editor: R. N. Moore

Infection and Immunity, February 2002, p. 544-560, Vol. 70, No. 2
0019-9567/01/$04.00+0     DOI: 10.1128/IAI.70.2.544-550.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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