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Infection and Immunity, February 2002, p. 620-630, Vol. 70, No. 2
0019-9567/01/$04.00+0     DOI: 70.2.620-630.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Fibronectin-Binding Proteins of Staphylococcus aureus Are Involved in Adherence to Human Airway Epithelium

Emmanuel Mongodin,1 Odile Bajolet,1,2 Jérôme Cutrona,1 Noël Bonnet,1 Florence Dupuit,1 Edith Puchelle,1 and Sophie de Bentzmann1*

INSERM UMRS514, IFR53, CHU Maison-Blanche,1 Laboratoire de Bactériologie-Virologie-Hygiène, CHU Robert Debré, 51092 Reims Cedex, France2

Received 17 May 2001/ Returned for modification 18 June 2001/ Accepted 1 November 2001

This study was designed to investigate the molecular mechanisms of Staphylococcus aureus adherence to human airway epithelium. Using a humanized bronchial xenograft model in the nude mouse and primary cultures of human airway epithelial cells (HAEC), we showed that S. aureus adhered mainly to undifferentiated HAEC whereas weak adherence (11- to 20-fold lower) to differentiated HAEC was observed (P < 0.01). A fibronectin (FN)-binding protein (FnBP)-deficient strain of S. aureus had a fivefold-lower adherence level to undifferentiated HAEC than did the parental strain (P < 0.005), suggesting that S. aureus FN-binding capacity is involved in the adherence to HAEC. We also showed that 97% of 32 S. aureus clinical strains, isolated from the airway secretions of cystic fibrosis patients (n = 18) and patients with nosocomial pneumonia (n = 14), possessed the two fnb genes. The strains from pneumonia patients had a significantly (P < 0.05) higher FN-binding capacity than did the strains from CF patients. This result was confirmed by the expression of FnBPs, investigated by Western ligand affinity blotting. Our results suggest a major role of FnBPs in the colonization of the airways by S. aureus and point to the importance of the adhesin regulatory pathways in the staphylococcal infectious process.

* Corresponding author. Present address: CNRS UPR 9027-IBSM, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France. Phone: 33 4 91164125. Fax: 33 4 91712124. E-mail: bentzman{at}ibsm.cnrs-mrs.fr.

Editor: E. I. Tuomanen

{dagger} Present address: Division of Infectious Diseases, Department of Medicine, Virginia Commonwealth University, Medical College of Virginia, Richmond, VA 23298-0049

Infection and Immunity, February 2002, p. 620-630, Vol. 70, No. 2
0019-9567/01/$04.00+0     DOI: 70.2.620-630.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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