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Infection and Immunity, February 2002, p. 787-793, Vol. 70, No. 2
0019-9567/01/$04.00+0     DOI: 70.2.787-793.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Phase Variation of Campylobacter jejuni 81-176 Lipooligosaccharide Affects Ganglioside Mimicry and Invasiveness In Vitro

Patricia Guerry,1* Christine M. Szymanski,1,{dagger} Martina M. Prendergast,2 Thomas E. Hickey,1 Cheryl P. Ewing,1 Dawn L. Pattarini,1 and Anthony P. Moran2

Enteric Diseases Program, Naval Medical Research Center, Silver Spring, Maryland 20910,1 Department of Microbiology, National University of Ireland Galway, Galway, Ireland2

Received 14 May 2001/ Returned for modification 2 August 2001/ Accepted 6 November 2001

The outer cores of the lipooligosaccharides (LOS) of many strains of Campylobacter jejuni mimic human gangliosides in structure. A population of cells of C. jejuni strain 81-176 produced a mixture of LOS cores which consisted primarily of structures mimicking GM2 and GM3 gangliosides, with minor amounts of structures mimicking GD1b and GD2. Genetic analyses of genes involved in the biosynthesis of the outer core of C. jejuni 81-176 revealed the presence of a homopolymeric tract of G residues within a gene encoding CgtA, an N-acetylgalactosaminyltransferase. Variation in the number of G residues within cgtA affected the length of the open reading frame, and these changes in cgtA corresponded to a change in LOS structure from GM2 to GM3 ganglioside mimicry. Site-specific mutation of cgtA in 81-176 resulted in a major LOS core structure that lacked GalNAc and resembled GM3 ganglioside. Compared to wild-type 81-176, the cgtA mutant showed a significant increase in invasion of INT407 cells. In comparison, a site-specific mutation of the neuC1 gene resulted in the loss of sialic acid in the LOS core and reduced resistance to normal human serum but had no affect on invasion of INT407 cells.

* Corresponding author. Mailing address: Enteric Diseases Program, Naval Medical Research Center, 503 Robert Grant Ave., Silver Spring, MD 20910-7500. Phone: (301) 319-7662. Fax: (301) 319-7679. E-mail: guerryp{at}nmrc.navy.mil.

Editor: V. J. DiRita

{dagger} Present address: Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada

Infection and Immunity, February 2002, p. 787-793, Vol. 70, No. 2
0019-9567/01/$04.00+0     DOI: 70.2.787-793.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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