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Infection and Immunity, February 2002, p. 988-992, Vol. 70, No. 2
0019-9567/01/$04.00+0     DOI: 10.1128/IAI.70.2.988-992.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Gene Expression Profiling in AGS Cells Stimulated with Helicobacter pylori Isogenic Strains (cagA Positive or cagA Negative)

Susanne Bach, Athanasios Makristathis, Manfred Rotter, and Alexander M. Hirschl*

Department of Clinical Microbiology, Hygiene Institute, University of Vienna, A-1090 Vienna, Austria

Received 13 June 2001/ Returned for modification 15 August 2001/ Accepted 20 October 2001

To study host response to CagA, human gastric cancer cell line AGS was infected with a Helicobacter pylori type I wild-type or isogenic cagA-negative mutant. Differentially expressed genes were identified using cDNA array technology. By Northern blotting, downregulation of focal adhesion kinase and upregulation of LIM kinase mRNA in the presence of CagA were clearly verified. Furthermore, upregulation of LIM kinase, macrophage inflammatory protein-2, c-myc, and bone morphogenetic protein-1 and downregulation of transcription factor Y-box binding protein-1 and focal adhesion kinase mRNA in response to H. pylori type I infection compared to the uninfected control could be shown by Northern blotting. Hence, these findings identified new targets for further functional studies on H. pylori-associated pathogenesis.

* Corresponding author. Mailing address: Department of Clinical Microbiology, Hygiene Institute, AKH-Wien, Waehringer Guertel 18-20/5P, A-1090 Vienna. Austria. Phone: 43 1 40 400 5154. Fax: 43 1 40 400 5228. E-mail: alexander.hirschl{at}univie.ac.at.

Editor: V. J. DiRita

Infection and Immunity, February 2002, p. 988-992, Vol. 70, No. 2
0019-9567/01/$04.00+0     DOI: 10.1128/IAI.70.2.988-992.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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