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Infection and Immunity, March 2002, p. 1113-1120, Vol. 70, No. 3
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.3.1113-1120.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Role of Glutathione Metabolism of Treponema denticola in Bacterial Growth and Virulence Expression

Lianrui Chu,^1* Zheng Dong,² Xiaoping Xu,¹ David L. Cochran,¹ and Jefferey L. Ebersole^1,3

Departments of Periodontics,¹ Pathology, University of Texas Health Science Center, San Antonio, Texas 78229 ,² Center for Oral Health Research, University of Kentucky, Lexington, Kentucky 40536³

Received 16 August 2001/ Returned for modification 24 October 2001/ Accepted 19 November 2001

Hydrogen sulfide (H₂S) is a major metabolic end product detected in deep periodontal pockets that is produced by resident periodontopathic microbiota associated with the progression of periodontitis. Treponema denticola, a member of the subgingival biofilm at disease sites, produces cystalysin, an enzyme that catabolizes cysteine, releasing H₂S. The metabolic pathway leading to H₂S formation in periodontal pockets has not been determined. We used a variety of thiol compounds as substrates for T. denticola to produce H₂S. Our results indicate that glutathione, a readily available thiol source in periodontal pockets, is a suitable substrate for H₂S production by this microorganism. In addition to H₂S, glutamate, glycine, ammonia, and pyruvate were metabolic end products of metabolism of glutathione. Cysteinyl glycine (Cys-Gly) was also catabolized by the bacteria, yielding glycine, H₂S, ammonia, and pyruvate. However, purified cystalysin could not catalyze glutathione and Cys-Gly degradation in vitro. Moreover, the enzymatic activity(ies) in T. denticola responsible for glutathione breakdown was inactivated by trypsin or proteinase K, by heating (56°C) and freezing (-20°C), by sonication, and by exposure to N-p-tosyl-L-lysine chloromethyl ketone (TLCK). These treatments had no effect on degradation of cysteine by the purified enzyme. In this study we delineated an enzymatic pathway for glutathione metabolism in the oral spirochete T. denticola; our results suggest that glutathione metabolism plays a role in bacterial nutrition and potential virulence expression.

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* Corresponding author. Mailing address: Department of Periodontics, University of Texas Health Science Center, San Antonio, TX 78229. Phone: (210) 567-6269. Fax: (210) 567-6858. E-mail: chul{at}uthscsa.edu.

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Infection and Immunity, March 2002, p. 1113-1120, Vol. 70, No. 3
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.3.1113-1120.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Chu, L., Lai, Y., Xu, X., Eddy, S., Yang, S., Song, L., Kolodrubetz, D. (2008). A 52-kDa Leucyl Aminopeptidase from Treponema denticola Is a Cysteinylglycinase That Mediates the Second Step of Glutathione Metabolism. J. Biol. Chem. 283: 19351-19358 [Abstract] [Full Text]  
• Lai, Y., Chu, L. (2008). Novel Mechanism for Conditional Aerobic Growth of the Anaerobic Bacterium Treponema denticola. Appl. Environ. Microbiol. 74: 73-79 [Abstract] [Full Text]  
• Chu, L., Xu, X., Dong, Z., Cappelli, D., Ebersole, J. L. (2003). Role for Recombinant {gamma}-Glutamyltransferase from Treponema denticola in Glutathione Metabolism. Infect. Immun. 71: 335-342 [Abstract] [Full Text]