This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Heuner, K. Articles by Hacker, J. Search for Related Content PubMed PubMed Citation Articles by Heuner, K. Articles by Hacker, J.

 Previous Article  |  Next Article 

Infection and Immunity, March 2002, p. 1604-1608, Vol. 70, No. 3
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.3.1604-1608.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Influence of the Alternative ²⁸ Factor on Virulence and Flagellum Expression of Legionella pneumophila

Institut für Molekulare Infektionsbiologie, Universität Würzburg, 97070 Würzburg, Germany

Received 14 June 2001/ Returned for modification 15 August 2001/ Accepted 20 November 2001

The fliA gene of Legionella pneumophila encoding the alternative ²⁸ factor was inactivated by introducing a kanamycin resistance cassette. Electron microscopy and Western blot analysis revealed that the fliA mutant strain is aflagellate and expresses no flagellin. Reporter gene assays indicated that the flaA promoter is not active in the fliA mutant strain. The fliA mutant strain multiplied less effectively in coculture with amoebae than the wild-type strain and was not able to replicate in coculture with Dictyostelium discoideum.

This article has been cited by other articles:

• Spirig, T., Tiaden, A., Kiefer, P., Buchrieser, C., Vorholt, J. A., Hilbi, H. (2008). The Legionella Autoinducer Synthase LqsA Produces an {alpha}-Hydroxyketone Signaling Molecule. J. Biol. Chem. 283: 18113-18123 [Abstract] [Full Text]  
• Vinzing, M., Eitel, J., Lippmann, J., Hocke, A. C., Zahlten, J., Slevogt, H., N'Guessan, P. D., Gunther, S., Schmeck, B., Hippenstiel, S., Flieger, A., Suttorp, N., Opitz, B. (2008). NAIP and Ipaf Control Legionella pneumophila Replication in Human Cells. J. Immunol. 180: 6808-6815 [Abstract] [Full Text]  
• Claret, L., Miquel, S., Vieille, N., Ryjenkov, D. A., Gomelsky, M., Darfeuille-Michaud, A. (2007). The Flagellar Sigma Factor FliA Regulates Adhesion and Invasion of Crohn Disease-associated Escherichia coli via a Cyclic Dimeric GMP-dependent Pathway. J. Biol. Chem. 282: 33275-33283 [Abstract] [Full Text]  
• Mampel, J., Spirig, T., Weber, S. S., Haagensen, J. A. J., Molin, S., Hilbi, H. (2006). Planktonic Replication Is Essential for Biofilm Formation by Legionella pneumophila in a Complex Medium under Static and Dynamic Flow Conditions. Appl. Environ. Microbiol. 72: 2885-2895 [Abstract] [Full Text]  
• Allard, K. A., Viswanathan, V. K., Cianciotto, N. P. (2006). lbtA and lbtB Are Required for Production of the Legionella pneumophila Siderophore Legiobactin. J. Bacteriol. 188: 1351-1363 [Abstract] [Full Text]  
• Goon, S., Ewing, C. P., Lorenzo, M., Pattarini, D., Majam, G., Guerry, P. (2006). A {sigma}28-Regulated Nonflagella Gene Contributes to Virulence of Campylobacter jejuni 81-176. Infect. Immun. 74: 769-772 [Abstract] [Full Text]  
• Dorschner, R. A., Lopez-Garcia, B., Peschel, A., Kraus, D., Morikawa, K., Nizet, V., Gallo, R. L. (2006). The mammalian ionic environment dictates microbial susceptibility to antimicrobial defense peptides. FASEB J. 20: 35-42 [Abstract] [Full Text]  
• Molofsky, A. B., Shetron-Rama, L. M., Swanson, M. S. (2005). Components of the Legionella pneumophila Flagellar Regulon Contribute to Multiple Virulence Traits, Including Lysosome Avoidance and Macrophage Death. Infect. Immun. 73: 5720-5734 [Abstract] [Full Text]  
• Bischoff, M., Dunman, P., Kormanec, J., Macapagal, D., Murphy, E., Mounts, W., Berger-Bachi, B., Projan, S. (2004). Microarray-Based Analysis of the Staphylococcus aureus {sigma}B Regulon. J. Bacteriol. 186: 4085-4099 [Abstract] [Full Text]  
• Soderberg, M. A., Rossier, O., Cianciotto, N. P. (2004). The Type II Protein Secretion System of Legionella pneumophila Promotes Growth at Low Temperatures. J. Bacteriol. 186: 3712-3720 [Abstract] [Full Text]  
• Bachman, M. A., Swanson, M. S. (2004). The LetE Protein Enhances Expression of Multiple LetA/LetS-Dependent Transmission Traits by Legionella pneumophila. Infect. Immun. 72: 3284-3293 [Abstract] [Full Text]  
• Bachman, M. A., Swanson, M. S. (2004). Genetic Evidence that Legionella pneumophila RpoS Modulates Expression of the Transmission Phenotype in Both the Exponential Phase and the Stationary Phase. Infect. Immun. 72: 2468-2476 [Abstract] [Full Text]  
• Jacobi, S., Schade, R., Heuner, K. (2004). Characterization of the Alternative Sigma Factor {sigma}54 and the Transcriptional Regulator FleQ of Legionella pneumophila, Which Are Both Involved in the Regulation Cascade of Flagellar Gene Expression. J. Bacteriol. 186: 2540-2547 [Abstract] [Full Text]