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Infection and Immunity, March 2002, p. 1615-1618, Vol. 70, No. 3
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.3.1615-1618.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Lipopolysaccharide-Specific but Not Anti-Flagellar Immunoglobulin A Monoclonal Antibodies Prevent Salmonella enterica Serotype Enteritidis Invasion and Replication within HEp-2 Cell Monolayers

Ianko D. Iankov,1 Dragomir P. Petrov,1 Ivan V. Mladenov,2 Iana H. Haralambieva,1 and Ivan G. Mitov1*

Department of Microbiology,1 Department of Biology, Medical University of Sofia, 1431 Sofia, Bulgaria2

Received 24 August 2001/ Returned for modification 1 November 2001/ Accepted 20 November 2001

The protective potential of immunoglobulin A (IgA) monoclonal antibodies (MAbs) directed against O and H antigens of Salmonella enterica serotype Enteritidis to prevent bacterial adhesion to and invasion of HEp-2 cells was evaluated. Although anti-flagellar IgA MAbs showed strong agglutinating capacities, they did not protect cell monolayers. In contrast, IgA MAbs specific for the O:9 epitope of Salmonella lipopolysaccharide antigen alone prevented S. enterica serotype Enteritidis entry and replication within HEp-2 cells, and the protection was not mediated by direct binding of antibodies to bacterial adhesins or by agglutination of microorganisms.

* Corresponding author. Mailing address: Department of Microbiology, Medical University of Sofia, Zdrave 2 St., 1431 Sofia, Bulgaria. Phone: 359 2 951 53 17. Fax: 359 2 952 03 45. E-mail: mitov{at}medfac.acad.bg.

Infection and Immunity, March 2002, p. 1615-1618, Vol. 70, No. 3
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.3.1615-1618.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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  • Forbes, S. J., Eschmann, M., Mantis, N. J. (2008). Inhibition of Salmonella enterica Serovar Typhimurium Motility and Entry into Epithelial Cells by a Protective Antilipopolysaccharide Monoclonal Immunoglobulin A Antibody. Infect. Immun. 76: 4137-4144 [Abstract] [Full Text]  


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