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Infection and Immunity, April 2002, p. 1860-1866, Vol. 70, No. 4
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.4.1860-1866.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Lactoferrin Inhibits the Lipopolysaccharide-Induced Expression and Proteoglycan-Binding Ability of Interleukin-8 in Human Endothelial Cells

Elisabeth Elass,* Maryse Masson, Joël Mazurier, and Dominique Legrand

Laboratoire de Chimie Biologique et Unité Mixte de Recherche no. 8576 du Centre National de la Recherche Scientifique, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France

Received 10 July 2001/ Returned for modification 24 September 2001/ Accepted 16 January 2002

Interleukin-8 (IL-8), a C-X-C chemokine bound to endothelium proteoglycans, initiates the activation and selective recruitment of leukocytes at inflammatory foci. We demonstrate that human lactoferrin, an antimicrobial lipopolysaccharide (LPS)-binding protein, decreases both IL-8 mRNA and protein expression induced by the complex Escherichia coli 055:B5 LPS/sCD14 in human umbilical vein endothelial cells. The use of recombinant lactoferrins mutated in the LPS-binding sites indicates that this inhibitory effect is mediated by an interaction of lactoferrin with LPS and CD14s that suppresses the endotoxin biological activity. Furthermore, since dimeric IL-8 and lactoferrin are both proteoglycan-binding molecules, the competition between these proteins for heparin binding was investigated. Lactoferrin strongly inhibited the interaction of radiolabeled IL-8 to immobilized heparin, whereas a lactoferrin variant lacking the amino acid residues essential for heparin binding was not inhibitory. Moreover, this process is specific, since serum transferrin, a glycoprotein whose structure is close to that of lactoferrin, did not prevent the interaction of IL-8 with heparin. These results suggest that the anti-inflammatory properties of lactoferrin during septicemia are related, at least in part, to the regulation of IL-8 production and also to the ability of lactoferrin to compete with chemokines for their binding to proteoglycans.

* Corresponding author. Mailing address: Laboratoire de Chimie Biologique, UMR CNRS no. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France. Phone: 33 3 20 33 72 39. Fax: 33 3 20 43 65 55. E-mail: Elisabeth.Elass{at}univ-lille1.fr.

Editor: R. N. Moore

Infection and Immunity, April 2002, p. 1860-1866, Vol. 70, No. 4
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.4.1860-1866.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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