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Infection and Immunity, April 2002, p. 2233-2237, Vol. 70, No. 4
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.4.2233-2237.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Complementation of a Treponema denticola flgE Mutant with a Novel Coumermycin A1-Resistant T. denticola Shuttle Vector System

Bo Chi,1 Ronald J. Limberger,2 and Howard K. Kuramitsu1*

Department of Oral Biology, State University of New York at Buffalo, Buffalo, New York 14214,1 David Axelrod Institute of Public Health, New York State Department of Health, Albany, New York 122012

Received 7 November 2001/ Returned for modification 28 December 2001/ Accepted 18 January 2002

By using the mutated gyrB gene from a spontaneous coumermycin A1-resistant Treponema denticola, an Escherichia coli-T. denticola shuttle vector that renders T. denticola resistant to coumermycin was constructed. The complete T. denticola flgE gene was cloned into the shuttle vector pKMCou, and the vector was transformed into the T. denticola ATCC 33520 flgE erythromycin-resistant knockout mutant HL210. The coumermycin-resistant transformants were motile and restored FlgE activity. This complementation system should prove useful in studying the virulence factors of T. denticola and uncultivable pathogenic spirochetes.

* Corresponding author. Mailing address: Department of Oral Biology, SUNY at Buffalo, 3435 Main St., Buffalo, NY 14214-3092. Phone: (716) 829-2068. Fax: (716) 829-3942. E-mail: kuramits{at}buffalo.edu.

Editor: E. I. Tuomanen

Infection and Immunity, April 2002, p. 2233-2237, Vol. 70, No. 4
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.4.2233-2237.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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