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Infection and Immunity, April 2002, p. 2238-2241, Vol. 70, No. 4
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.4.2238-2241.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Gel Shift Assay of Nuclear Extracts from Histoplasma capsulatum Demonstrates the Presence of Several DNA Binding Proteins

Atanas Ignatov and Elizabeth J. Keath*

Department of Biology, Saint Louis University, St. Louis, Missouri 63103

Received 28 September 2001/ Returned for modification 9 November 2001/ Accepted 18 January 2002

A gel shift assay was optimized to detect several general DNA binding proteins from Histoplasma capsulatum strain G217B. The electrophoretic mobility shift assay (EMSA) technique also detected protein(s) recognizing a pyrimidine-rich motif found in several Histoplasma promoters. Establishment of EMSA conditions provides an important framework to evaluate regulation of homeostatic or phase-specific genes that may influence virulence in Histoplasma and other dimorphic fungal pathogens.

* Corresponding author. Mailing address: Department of Biology, 3507 Laclede Ave., Saint Louis University, St. Louis, MO 63103-2010. Phone: (314) 977-3976. Fax: (314) 977-3658. E-mail: keath{at}slu.edu.

Editor: T. R. Kozel

Infection and Immunity, April 2002, p. 2238-2241, Vol. 70, No. 4
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.4.2238-2241.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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