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Infection and Immunity, May 2002, p. 2351-2360, Vol. 70, No. 5
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.5.2351-2360.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Genome-Based Identification of Chromosomal Regions Specific for Salmonella spp.

Imke Hansen-Wester and Michael Hensel*

Institut für Klinische Mikrobiologie, Immunologie und Hygiene, Friedrich-Alexander Universität Erlangen-Nürnberg, Erlangen, Germany

Received 7 September 2001/ Returned for modification 31 October 2001/ Accepted 31 January 2002

Acquisition of genomic elements by horizontal gene transfer represents an important mechanism in the evolution of bacterial species. Pathogenicity islands are a subset of horizontally acquired elements present in various pathogens. These elements are frequently located adjacent to tRNA genes. We performed a comparative genome analysis of Salmonella enterica serovars Typhi and Typhimurium and Escherichia coli and scanned tRNA loci for the presence of species-specific, horizontally acquired genomic elements. A large number of species-specific elements were identified. Here, we describe the characteristics of four large chromosomal insertions at tRNA genes of Salmonella spp. The tRNA-associated elements harbor various genes previously identified as single virulence genes, indicating that these genes have been acquired with large chromosomal insertions. Southern blot analyses confirmed that the tRNA-associated elements are specific to Salmonella and also indicated a heterogeneous distribution within the salmonellae. Systematic scanning for insertions at tRNA genes thus represents a tool for the identification of novel pathogenicity islands.

* Corresponding author. Mailing address: Institut für Klinische Mikrobiologie, Immunologie und Hygiene, Universität Erlangen-Nürnberg, Wasserturmstr. 3-5, D-91054 Erlangen, Germany. Phone: 49 (0)9131 85 23640. Fax: 49 (0)9131 85 22531. E-mail: hensel{at}mikrobio.med.uni-erlangen.de.

Editor: B. B. Finlay

Infection and Immunity, May 2002, p. 2351-2360, Vol. 70, No. 5
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.5.2351-2360.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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