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Infection and Immunity, May 2002, p. 2368-2374, Vol. 70, No. 5
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.5.2368-2374.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Dendritic Cells as Effector Cells: Gamma Interferon Activation of Murine Dendritic Cells Triggers Oxygen-Dependent Inhibition of Toxoplasma gondii Replication

F. Aline, D. Bout, and I. Dimier-Poisson*

UMR Université-INRA d'Immunologie Parasitaire, UFR des Sciences Pharmaceutiques, 37200 Tours, France

Received 15 October 2001/ Returned for modification 20 November 2001/ Accepted 1 February 2002

Toxoplasma gondii is an obligate intracellular parasite that infects a wide variety of nucleated cells in its numerous intermediate hosts, including humans. Much interest has focused on the ability of gamma interferon (IFN-{gamma})-activated macrophages to prevent intracellular replication, but some other cells (e.g., fibroblasts, endothelial cells, microglial cells, astrocytes, enterocytes and retinal pigment cells) can also be activated to induce this inhibition of proliferation. Dendritic cells are generally known to be involved in the induction of immune responses, but no previous study had investigated the possibility that dendritic cells may act as effector cells of this system. Our results show that IFN-{gamma}-activation inhibits the replication of T. gondii in dendritic cells, with the inhibition being dose dependent. Neither nitrogen derivatives nor tryptophan starvation appears to be involved in the inhibition of parasite replication by IFN-{gamma}. Experiments with oxygen scavengers indicate that intracellular T. gondii replication is oxygen dependent. Our findings suggest that, in addition to their essential role in stimulating the immune system, dendritic cells probably act as effector cells in the first line of defense against pathogen invasion.

* Corresponding author. Mailing address: UFR des Sciences Pharmaceutiques, 31 Ave. Monge, 37200 Tours, France. Phone: 33-247-36-71-84. Fax: 33-247-36-72-52. E-mail: dimier{at}univ-tours.fr

Editor: W. A. Petri, Jr.

Infection and Immunity, May 2002, p. 2368-2374, Vol. 70, No. 5
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.5.2368-2374.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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