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Infection and Immunity, May 2002, p. 2408-2413, Vol. 70, No. 5
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.5.2408-2413.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

The Group B Streptococcal C5a Peptidase Is Both a Specific Protease and an Invasin

Qi Cheng, Deborah Stafslien, Sai Sudha Purushothaman, and Patrick Cleary*

Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455

Received 26 November 2001/ Returned for modification 14 January 2002/ Accepted 7 February 2002

The group B streptococcus (GBS) is a major cause of pneumonia, sepsis, and meningitis in neonates and a serious cause of mortality or morbidity in immunocompromised adults. Although these streptococci adhere efficiently and invade a variety of tissue-specific epithelial and endothelial cells, adhesins and invasins are still unknown. All serotypes of GBS studied to date express C5a peptidase (SCPB) on their surface. This investigation addresses the possibility that this relatively large surface protein has additional activities. Rabbit anti-SCPB serum inhibited invasion of lung epithelial A549 cells by the serotype Ia strain O90R, suggesting that SCPB is an invasin. This was confirmed by inserting an in-frame 25-amino-acid deletion into the scpB gene. Invasion of HEp2 and A549 human cell lines was significantly reduced by the mutation. Enzyme-linked immunosorbent assays were used to demonstrate that purified SCPB protein binds directly to HEp2 and A549 cells and also binds the extracellular matrix protein fibronectin. Binding was dose dependent and saturable. These results suggested that SCPB is one of several potential invasins essential for GBS colonization of damaged epithelium.

* Corresponding author. Mailing address: Department of Microbiology, MMC 196, Minneapolis, MN 55455. Phone: (612) 624-3932. Fax: (612) 626-0623. E-mail: Cleary{at}lenti.med.umn.edu.

Editor: E. I. Tuomanen

Infection and Immunity, May 2002, p. 2408-2413, Vol. 70, No. 5
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.5.2408-2413.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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