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Infection and Immunity, May 2002, p. 2414-2418, Vol. 70, No. 5
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.5.2414-2418.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Vaccination of Pregnant Dams with IntiminO157 Protects Suckling Piglets from Escherichia coli O157:H7 Infection

Evelyn A. Dean-Nystrom,1* Lisa J. Gansheroff,2 Melody Mills,2 Harley W. Moon,3 and Alison D. O'Brien2

Pre-Harvest Food Safety and Enteric Diseases Research Unit, National Animal Disease Center, U.S. Department of Agriculture, Agricultural Research Service, Ames, Iowa 50010,1 Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814,2 Iowa State University, Ames, Iowa 500113

Received 13 November 2001/ Returned for modification 19 December 2001/ Accepted 13 February 2002

Cattle are important reservoirs of enterohemorrhagic Escherichia coli (EHEC) O157:H7 that cause disease in humans. Both dairy and beef cattle are asymptomatically and sporadically infected with EHEC. Our long-term goal is to develop an effective vaccine to prevent cattle from becoming infected and transmitting EHEC O157:H7 to humans. We used passive immunization of neonatal piglets (as a surrogate model) to determine if antibodies against EHEC O157 adhesin (intiminO157) inhibit EHEC colonization. Pregnant swine (dams) with serum anti-intimin titers of <=100 were vaccinated twice with purified intiminO157 or sham-vaccinated with sterile buffer. IntiminO157-specific antibody titers in colostrum and serum of dams were increased after parenteral vaccination with intiminO157. Neonatal piglets were allowed to suckle vaccinated or sham-vaccinated dams for up to 8 h before they were inoculated with 106 CFU of a Shiga toxin-negative (for humane reasons) strain of EHEC O157:H7. Piglets were necropsied at 2 to 10 days after inoculation, and intestinal samples were collected for determination of bacteriological counts and histopathological analysis. Piglets that ingested colostrum containing intiminO157-specific antibodies from vaccinated dams, but not those nursing sham-vaccinated dams, were protected from EHEC O157:H7 colonization and intestinal damage. These results establish intiminO157 as a viable candidate for an EHEC O157:H7 antitransmission vaccine.

* Corresponding author. Mailing address: USDA, ARS, National Animal Disease Center, P.O. Box 70, Ames, IA 50010-0070. Phone: (515) 663-7376. Fax: (515) 663-7458. E-mail: enystrom{at}nadc.ars.usda.gov.

Editor: J. D. Clements

Infection and Immunity, May 2002, p. 2414-2418, Vol. 70, No. 5
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.5.2414-2418.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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