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Infection and Immunity, May 2002, p. 2670-2675, Vol. 70, No. 5
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.5.2670-2675.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Fibronectin Attachment Protein Is Necessary for Efficient Attachment and Invasion of Epithelial Cells by Mycobacterium avium subsp. paratuberculosis

Department of Veterinary Pathobiology, Purdue University, West Lafayette, Indiana 47907

Received 3 August 2001/ Returned for modification 27 September 2001/ Accepted 22 January 2002

Attachment and ingestion of Mycobacterium avium subsp. paratuberculosis by two epithelial cell lines were enhanced by soluble fibronectin (FN). Peptide blocking of the FN attachment protein (FAP-P) inhibited the internalization of M. avium subsp. paratuberculosis. Disruption of FAP-P expression significantly reduced attachment and ingestion of M. avium subsp. paratuberculosis by T-24 and Caco-2 cells. The results indicate that the interaction between FN and FAP-P facilitates attachment and internalization of M. avium subsp. paratuberculosis by epithelial cells.

Editor: V. J. DiRita

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