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Infection and Immunity, May 2002, p. 2681-2689, Vol. 70, No. 5
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.5.2681-2689.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Afa, a Diffuse Adherence Fibrillar Adhesin Associated with Enteropathogenic Escherichia coli

Rogéria Keller,1* Juana G. Ordoñez,1 Rosana R. de Oliveira,1 Luiz R. Trabulsi,1 Thomas J. Baldwin,2 and Stuart Knutton3

Laboratório Especial de Microbiologia, Instituto Butantan, São Paulo, Brazil,1 Institute of Infections and Immunity, Nottingham University, Nottingham,2 Institute of Child Health, Birmingham University, Birmingham, United Kingdom3

Received 2 July 2001/ Returned for modification 31 October 2001/ Accepted 8 January 2002

O55 is one of the most frequent enteropathogenic Escherichia coli (EPEC) O serogroups implicated in infantile diarrhea in developing countries. Multilocus enzyme electrophoresis analysis showed that this serogroup includes two major electrophoretic types (ET), designated ET1 and ET5. ET1 corresponds to typical EPEC, whilst ET5 comprises strains with different combinations of virulence genes, including those for localized adherence (LA) and diffuse adherence (DA). Here we report that ET5 DA strains possess a DA adhesin, designated EPEC Afa. An 11.6-kb chromosomal region including the DA adhesin operon from one O55:H- ET5 EPEC strain was sequenced and found to encode a protein with 98% identity to AfaE-1, an adhesin associated with uropathogenic E. coli. Although described as an afimbrial adhesin, we show that both AfaE-1 and EPEC Afa possess fine fibrillar structures. This is the first characterization and demonstration of an Afa adhesin associated with EPEC.

* Corresponding author. Mailing address: Laboratório Especial de Microbiologia, Prédio Novo 2° andar, Instituto Butatan, Av. Vital Brasil, 1500, 05503-900 São Paulo, SP, Brazil. Phone: 55 11 3726 7222, ext. 2075. Fax: 55 11 3726 1505. E-mail: keller{at}usp.br.

Editor: A. D. O'Brien

Infection and Immunity, May 2002, p. 2681-2689, Vol. 70, No. 5
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.5.2681-2689.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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