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Infection and Immunity, May 2002, p. 2715-2720, Vol. 70, No. 5
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.5.2715-2720.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Differential Persistence among Genomovars of the Burkholderia cepacia Complex in a Murine Model of Pulmonary Infection

Karen K. Chu,1 Donald J. Davidson,2 T. Keith Halsey,1 Jacqueline W. Chung,1 and David P. Speert1,2*

Departments of Paediatrics and Pathology,1 Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada2

Received 27 August 2001/ Returned for modification 21 November 2001/ Accepted 11 February 2002

Cystic fibrosis patients infected with strains from different genomovars of the Burkholderia cepacia complex can experience diverse clinical outcomes. To identify genomovar-specific determinants that might be responsible for these differences, we developed a pulmonary model of infection in BALB/c mice. Mice were rendered leukopenic by administration of cyclophosphamide prior to intranasal challenge with 1.6 x 104 bacteria. Five of six genomovar II strains persisted at stable numbers in the lungs until day 16 with minimal toxicity, whereas zero of seven genomovar III strains persisted but resulted in variable toxicity. We have developed a chronic pulmonary model of B. cepacia infection which reveals differences among genomovars in terms of clinical infection outcome.

* Corresponding author. Mailing address: British Columbia Research Institute for Children's and Women's Health, 950 W. 28th Ave., Rm. 375, Vancouver, British Columbia, Canada V5Z 4H4. Phone: (604) 875-2438. Fax: (604) 875-2226. E-mail: speert{at}interchange.ubc.ca.

Editor: V. J. DiRita

Infection and Immunity, May 2002, p. 2715-2720, Vol. 70, No. 5
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.5.2715-2720.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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