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Infection and Immunity, May 2002, p. 2730-2733, Vol. 70, No. 5
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.5.2730-2733.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Autonomous Expression of the slo Gene of the Bicistronic nga-slo Operon of Streptococcus pyogenes

Dragutin J. Savic,* William M. McShan, and Joseph J. Ferretti

Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73190

Received 2 October 2001/ Returned for modification 21 December 2001/ Accepted 7 February 2002

A recent model for cytolysin-mediated translocation in Streptococcus pyogenes proposes that NAD-glycohydrolase is translocated through streptolysin O-generated pores into a host cell (J. Madden, N. Ruiz, and M. Caparon, Cell 104:143-152, 2001). This model also assumes that the NAD-glycohydrolase (nga) and streptolysin O (slo) genes that code for these products are organized in an operon-like structure expressed from a single promoter only (nga). We expand this model by showing that slo possesses its own autonomous promoter, which is located 155 bp upstream of the slo gene. Under experimental conditions in which S. pyogenes is grown in THY medium, the strength of the slo promoter, as measured by the activity of a lacZ reporter gene, resulted in low but highly reproducible values. Finally, we demonstrated that sloR, a S. pyogenes gene that closely resembles the Clostridium perfringens pfoR gene, exerts a negative effect on the expression of the slo gene.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, 940 S. L. Young Blvd., Oklahoma City, OK 73104. Phone: (405) 271-1202. Fax: (405) 271-3117. E-mail: Dragutin-Savic{at}ouhsc.edu.

Editor: E. I. Toumanen

Infection and Immunity, May 2002, p. 2730-2733, Vol. 70, No. 5
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.5.2730-2733.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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