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Infection and Immunity, May 2002, p. 2734-2738, Vol. 70, No. 5
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.5.2734-2738.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

A Lipid Core Peptide Construct Containing a Conserved Region Determinant of the Group A Streptococcal M Protein Elicits Heterologous Opsonic Antibodies

Colleen Olive,1* Michael R. Batzloff,1 Anikó Horváth,2 Allan Wong,2 Timothy Clair,1 Penny Yarwood,1 Istvan Toth,2 and Michael F. Good1

Division of Infectious Diseases and Immunology, Cooperative Research Centre for Vaccine Technology, The Queensland Institute of Medical Research, Brisbane, Queensland 4029,1 School of Pharmacy, The University of Queensland, Brisbane, Queensland 4072, Australia2

Received 29 November 2001/ Returned for modification 22 January 2002/ Accepted 13 February 2002

The study reported here investigated the immunogenicity and protective potential of a lipid core peptide (LCP) construct containing a conserved region determinant of M protein, defined as peptide J8. Parenteral immunization of mice with LCP-J8 led to the induction of high-titer serum immunoglobulin G J8-specific antibodies when the construct was coadministered with complete Freund's adjuvant (CFA) or administered alone. LCP-J8 in CFA had significantly enhanced immunogenicity compared with the monomeric peptide J8 given in CFA. Moreover, LCP-J8/CFA and LCP-J8 antisera opsonized four different group A streptococcal (GAS) strains, and the antisera did not cross-react with human heart tissue proteins. These data indicate the potential of an LCP-based M protein conserved region GAS vaccine in the induction of broadly protective immune responses in the absence of a conventional adjuvant.

* Corresponding author. Mailing address: Division of Infectious Diseases and Immunology, The Queensland Institute of Medical Research, PO Royal Brisbane Hospital, Brisbane, QLD 4029, Australia. Phone: 61-73362 0431. Fax: 61-7-3362 0104. E-mail: colleenO{at}qimr.edu.au.

Editor: J. T. Barbieri

Infection and Immunity, May 2002, p. 2734-2738, Vol. 70, No. 5
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.5.2734-2738.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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