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Infection and Immunity, June 2002, p. 3073-3079, Vol. 70, No. 6
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.6.3073-3079.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Helicobacter pylori Does Not Require Lewis X or Lewis Y Expression To Colonize C3H/HeJ mice

Tohru Takata,1,2* Emad El-Omar,1,3 Margarita Camorlinga,1,4 Stuart A. Thompson,1,5 Yutaka Minohara,6 Peter B. Ernst,6 and Martin J. Blaser1,2,7

Division of Infectious Diseases, Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee,1 Department of Medicine, New York University School of Medicine,2 Department of Veterans Affairs Medical Center, New York, New York,7 Department of Medicine and Therapeutics, Aberdeen University, Aberdeen, United Kingdom,3 Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta, Georgia,5 Department of Internal Medicine, University of Virginia, Charlottesville, Virginia,6 Unidad de Investigacion de Enfermedades Infecciosas, Hospital de Pediatria, Instituto Mexicano del Seguro Social, Mexico City, Mexico4

Received 1 October 2001/ Returned for modification 13 December 2001/ Accepted 25 February 2002

Helicobacter pylori strains frequently express Lewis X (Lex) and/or Ley on their cell surfaces as constituents of the O antigens of their lipopolysaccharide molecules. To assess the effect of Lex and Ley expression on the ability of H. pylori to colonize the mouse stomach and to adhere to epithelial cells, isogenic mutants were created in which fucT1 alone or fucT1 and fucT2, which encode the fucosyl transferases necessary for Lex and Ley expression, were deleted. C3H/HeJ mice were experimentally challenged with either wild-type 26695 H. pylori or its isogenic mutants. All strains, whether passaged in the laboratory or recovered after mouse passage, colonized the mice well and without consistent differences. During colonization by the mutants, there was no reversion to wild type. Similarly, adherence to AGS and KatoIII cells was unaffected by the mutations. Together, these findings indicate that Le expression is not necessary for mouse gastric colonization or for H. pylori adherence to epithelial cells.

* Corresponding author. Permanent address: First Department of Internal Medicine, School of Medicine, Fukuoka University, 7-45-1 Nanakuma, Jonan-Ku, Fukuoka 814-0180, Japan. Phone: 81(92)801-1011. Fax: 81(92)865-5656. E-mail: takatt01{at}med.nyu.edu.

Editor: V. J. DiRita

Infection and Immunity, June 2002, p. 3073-3079, Vol. 70, No. 6
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.6.3073-3079.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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