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Infection and Immunity, June 2002, p. 3180-3186, Vol. 70, No. 6
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.6.3180-3186.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Expression of the Biofilm-Associated Protein Interferes with Host Protein Receptors of Staphylococcus aureus and Alters the Infective Process

Carme Cucarella,1 M. Ángeles Tormo,1 Erwin Knecht,2 Beatriz Amorena,3 Íñigo Lasa,3 Timothy J. Foster,4 and José R. Penadés1*

Unit of Biochemistry, Department of Basic Biomedical Science, Cardenal Herrera-C. E. U. University, 46113 Moncada, Valencia,1 Instituto de Investigaciones Citológicas, FVIB, 46010 Valencia,2 Instituto de Agrobiotecnología y Recursos Naturales, CSIC-Universidad Pública de Navarra, 31006 Pamplona, Navarra, Spain,3 Microbiology Department, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland4

Received 24 October 2001/ Returned for modification 17 December 2001/ Accepted 26 February 2002

The adherence of Staphylococcus aureus to soluble proteins and extracellular-matrix components of the host is one of the key steps in the pathogenesis of staphylococcal infections. S. aureus presents a family of adhesins called MSCRAMMs (microbial surface components recognizing adhesive matrix molecules) that specifically recognize host matrix components. We examined the influence of biofilm-associated protein (Bap) expression on S. aureus adherence to host proteins, epithelial cell cultures, and mammary gland sections and on colonization of the mammary gland in an in vivo infection model. Bap-positive strain V329 showed lower adherence to immobilized fibrinogen and fibronectin than isogenic Bap-deficient strain m556. Bacterial adherence to histological sections of mammary gland and bacterial internalization into 293 cells were significantly lower in the Bap-positive strains. In addition, the Bap-negative strain showed significantly higher colonization in vivo of sheep mammary glands than the Bap-positive strain. Taken together, these results strongly suggest that the expression of the Bap protein interferes with functional properties of the MSCRAMM proteins, preventing initial bacterial attachment to host tissues and cellular internalization.

* Corresponding author. Mailing address: Veterinary School, Cardenal Herrera-C. E. U. University, Edificio Seminario, 46113 Moncada, Valencia, Spain. Phone: 34-96-1369000. Fax: 34-96-1395272. E-mail: jpenades{at}uch.ceu.es.

Editor: E. I. Tuomanen

Infection and Immunity, June 2002, p. 3180-3186, Vol. 70, No. 6
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.6.3180-3186.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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