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Infection and Immunity, June 2002, p. 3259-3263, Vol. 70, No. 6
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.6.3259-3263.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Entamoeba histolytica Lectins Contain Unique 6-Cys or 8-Cys Chitin-Binding Domains

Katrina Van Dellen,1 Sudip K. Ghosh,1 Phillips W. Robbins,2 Brendan Loftus,3 and John Samuelson1*

Department of Immunology and Infectious Diseases, Harvard School of Public Health,1 Department of Cell Biology, Boston University School of Dental Medicine, Boston, Massachusetts,2 The Institute for Genomic Research, Rockville, Maryland3

Received 3 August 2001/ Returned for modification 1 October 2001/ Accepted 22 February 2002

The Jacob lectin, the most abundant glycoprotein in the cyst wall of Entamoeba invadens, contains five unique 6-Cys chitin-binding domains (CBDs). We identified Entamoeba histolytica and Entamoeba dispar genes encoding Jacob homologues, each of which contains two predicted 6-Cys CBDs. A unique 8-Cys CBD was found at the N termini of the E. histolytica chitinase and three other predicted lectins, called Jessie 1 to Jessie 3. The CBDs of four E. histolytica lectins (Jacob, chitinase, and Jessies 2 and 3) were expressed in secretory vesicles of transfected amebae and shown to bind to particulate chitin.

* Corresponding author. Mailing address: Department of Immunology and Infectious Diseases, Harvard School of Public Health, 665 Huntington Ave., Boston, MA 02115. Phone: (617) 432-4670. Fax: (617) 738-4914. E-mail: jsamuels{at}hsph.harvard.edu.

Editor: J. M. Mansfield

Infection and Immunity, June 2002, p. 3259-3263, Vol. 70, No. 6
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.6.3259-3263.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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