This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Brumell, J. H.
Right arrow Articles by Finlay, B. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brumell, J. H.
Right arrow Articles by Finlay, B. B.

 Previous Article  |  Next Article 

Infection and Immunity, June 2002, p. 3264-3270, Vol. 70, No. 6
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.6.3264-3270.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Disruption of the Salmonella-Containing Vacuole Leads to Increased Replication of Salmonella enterica Serovar Typhimurium in the Cytosol of Epithelial Cells

John H. Brumell, Patrick Tang, Michelle L. Zaharik, and B. Brett Finlay*

Biotechnology Laboratory and Departments of Biochemistry and Molecular Biology, Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, V6T 1Z3, Canada

Received 11 October 2001/ Returned for modification 5 January 2002/ Accepted 10 March 2002

Salmonella enterica serovar Typhimurium is a facultative intracellular pathogen that inhabits a vacuolar compartment, called the Salmonella-containing vacuole (SCV), in infected host cells. Maintenance of the SCV is accomplished by SifA, and mutants of this Salmonella pathogenicity island 2 type III effector replicate more efficiently in epithelial cells. Here we demonstrate that enhanced replication of sifA mutants occurs in the cytosol of these cells. Increased replication of wild-type bacteria was also observed in cells treated with wortmannin or expressing Rab5 Q79L or Rab7 N125I, all of which caused a loss of SCV integrity. Our findings demonstrate the requirement of the host cell endosomal system for maintenance of the SCV and that loss of this compartment allows increased replication of serovar Typhimurium in the cytosol of epithelial cells.

* Corresponding author. Mailing address: Biotechnology Laboratory, University of British Columbia, Room 237, Wesbrook Building, 6174 University Blvd., Vancouver, BC, Canada V6T 1Z3. Phone: (604) 822-2210. Fax: (604) 822-9830. E-mail: bfinlay{at}interchange.ubc.ca.

Editor: A. D. O'Brien

Infection and Immunity, June 2002, p. 3264-3270, Vol. 70, No. 6
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.6.3264-3270.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Noda, T., Yoshimori, T. (2009). Molecular basis of canonical and bactericidal autophagy. Int Immunol 21: 1199-1204 [Abstract] [Full Text]  
  • Albaghdadi, H., Robinson, N., Finlay, B., Krishnan, L., Sad, S. (2009). Selectively Reduced Intracellular Proliferation of Salmonella enterica Serovar Typhimurium within APCs Limits Antigen Presentation and Development of a Rapid CD8 T Cell Response. J. Immunol. 183: 3778-3787 [Abstract] [Full Text]  
  • Wasylnka, J. A., Bakowski, M. A., Szeto, J., Ohlson, M. B., Trimble, W. S., Miller, S. I., Brumell, J. H. (2008). Role for Myosin II in Regulating Positioning of Salmonella-Containing Vacuoles and Intracellular Replication. Infect. Immun. 76: 2722-2735 [Abstract] [Full Text]  
  • Molmeret, M., Santic', M., Asare, R., Carabeo, R. A., Kwaik, Y. A. (2007). Rapid Escape of the dot/icm Mutants of Legionella pneumophila into the Cytosol of Mammalian and Protozoan Cells. Infect. Immun. 75: 3290-3304 [Abstract] [Full Text]  
  • Appelberg, R. (2006). Macrophage nutriprive antimicrobial mechanisms. J. Leukoc. Biol. 79: 1117-1128 [Abstract] [Full Text]  
  • Birmingham, C. L., Smith, A. C., Bakowski, M. A., Yoshimori, T., Brumell, J. H. (2006). Autophagy Controls Salmonella Infection in Response to Damage to the Salmonella-containing Vacuole. J. Biol. Chem. 281: 11374-11383 [Abstract] [Full Text]  
  • Zhang, Y., Higashide, W., Dai, S., Sherman, D. M., Zhou, D. (2005). Recognition and Ubiquitination of Salmonella Type III Effector SopA by a Ubiquitin E3 Ligase, HsRMA1. J. Biol. Chem. 280: 38682-38688 [Abstract] [Full Text]  
  • Ohlson, M. B., Fluhr, K., Birmingham, C. L., Brumell, J. H., Miller, S. I. (2005). SseJ Deacylase Activity by Salmonella enterica Serovar Typhimurium Promotes Virulence in Mice. Infect. Immun. 73: 6249-6259 [Abstract] [Full Text]  
  • Birmingham, C. L., Jiang, X., Ohlson, M. B., Miller, S. I., Brumell, J. H. (2005). Salmonella-Induced Filament Formation Is a Dynamic Phenotype Induced by Rapidly Replicating Salmonella enterica Serovar Typhimurium in Epithelial Cells. Infect. Immun. 73: 1204-1208 [Abstract] [Full Text]  
  • Lyons, S., Wang, L., Casanova, J. E., Sitaraman, S. V., Merlin, D., Gewirtz, A. T. (2004). Salmonella typhimurium transcytoses flagellin via an SPI2-mediated vesicular transport pathway. J. Cell Sci. 117: 5771-5780 [Abstract] [Full Text]  
  • Drecktrah, D., Knodler, L. A., Steele-Mortimer, O. (2004). Modulation and Utilization of Host Cell Phosphoinositides by Salmonella spp.. Infect. Immun. 72: 4331-4335 [Full Text]  
  • Molmeret, M., Bitar, D. M., Han, L., Kwaik, Y. A. (2004). Disruption of the Phagosomal Membrane and Egress of Legionella pneumophila into the Cytoplasm during the Last Stages of Intracellular Infection of Macrophages and Acanthamoeba polyphaga. Infect. Immun. 72: 4040-4051 [Abstract] [Full Text]  
  • Harrison, R. E., Brumell, J. H., Khandani, A., Bucci, C., Scott, C. C., Jiang, X., Finlay, B. B., Grinstein, S. (2004). Salmonella Impairs RILP Recruitment to Rab7 during Maturation of Invasion Vacuoles. Mol. Biol. Cell 15: 3146-3154 [Abstract] [Full Text]  
  • Guignot, J., Caron, E., Beuzon, C., Bucci, C., Kagan, J., Roy, C., Holden, D. W. (2004). Microtubule motors control membrane dynamics of Salmonella-containing vacuoles. J. Cell Sci. 117: 1033-1045 [Abstract] [Full Text]  
  • Freeman, J. A., Ohl, M. E., Miller, S. I. (2003). The Salmonella enterica Serovar Typhimurium Translocated Effectors SseJ and SifB Are Targeted to the Salmonella-Containing Vacuole. Infect. Immun. 71: 418-427 [Abstract] [Full Text]  
  • Beuzon, C. R., Salcedo, S. P., Holden, D. W. (2002). Growth and killing of a Salmonella enterica serovar Typhimurium sifA mutant strain in the cytosol of different host cell lines. Microbiology 148: 2705-2715 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»