This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tada, H. Articles by Takada, H. Search for Related Content PubMed PubMed Citation Articles by Tada, H. Articles by Takada, H.

 Previous Article  |  Next Article 

Infection and Immunity, June 2002, p. 3304-3307, Vol. 70, No. 6
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.6.3304-3307.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Proteolysis of CD14 on Human Gingival Fibroblasts by Arginine-Specific Cysteine Proteinases from Porphyromonas gingivalis Leading to Down-Regulation of Lipopolysaccharide-Induced Interleukin-8 Production

Department of Microbiology and Immunology,¹ Department of Periodontics and Endodontics,² Department of Oral Biochemistry, Tohoku University School of Dentistry, Sendai 980-8575,³ Division of Molecular Pathology, Department of Neuroscience and Immunology, Kumamoto University Graduate School of Medical Science, Kumamoto 860-0811, Japan,⁴ Department of Microbiology and Immunology, Institute of Molecular Biology, Jagiellonian University, 31-120 Craców, Poland,⁵ Department of Biochemistry, University of Georgia, Athens, Georgia 30602⁶

Received 15 October 2001/ Returned for modification 11 December 2001/ Accepted 25 February 2002

Arginine-specific cysteine proteinases (gingipains-R) from periodontopathic Porphyromonas gingivalis cleaved CD14, a bacterial pattern recognition receptor, on human gingival fibroblasts (HGF). Consequently, gingipains-R reduced lipopolysaccharide-induced interleukin-8 production by HGF, indicating that gingipains-R inhibited CD14-dependent HGF activation and are involved in immune evasion by the bacterium in periodontal tissues.

Editor: J. D. Clements

This article has been cited by other articles:

• Uehara, A., Naito, M., Imamura, T., Potempa, J., Travis, J., Nakayama, K., Takada, H. (2008). Dual regulation of interleukin-8 production in human oral epithelial cells upon stimulation with gingipains from Porphyromonas gingivalis. J Med Microbiol 57: 500-507 [Abstract] [Full Text]  
• Allaker, R.P., Dymock, D. (2005). Proceedings of the 8th European Oral Microbiology Workshop. Adv. Dent. Res. 18: 27-33 [Full Text]  
• Uehara, A., Muramoto, K., Imamura, T., Nakayama, K., Potempa, J., Travis, J., Sugawara, S., Takada, H. (2005). Arginine-Specific Gingipains from Porphyromonas gingivalis Stimulate Production of Hepatocyte Growth Factor (Scatter Factor) through Protease-Activated Receptors in Human Gingival Fibroblasts in Culture. J. Immunol. 175: 6076-6084 [Abstract] [Full Text]  
• Mezyk-Kopec, R., Bzowska, M., Potempa, J., Bzowska, M., Jura, N., Sroka, A., Black, R. A., Bereta, J. (2005). Inactivation of Membrane Tumor Necrosis Factor Alpha by Gingipains from Porphyromonas gingivalis. Infect. Immun. 73: 1506-1514 [Abstract] [Full Text]  
• Andrian, E., Grenier, D., Rouabhia, M. (2004). In Vitro Models of Tissue Penetration and Destruction by Porphyromonas gingivalis. Infect. Immun. 72: 4689-4698 [Abstract] [Full Text]  
• Coats, S. R., Reife, R. A., Bainbridge, B. W., Pham, T.-T. T., Darveau, R. P. (2003). Porphyromonas gingivalis Lipopolysaccharide Antagonizes Escherichia coli Lipopolysaccharide at Toll-Like Receptor 4 in Human Endothelial Cells. Infect. Immun. 71: 6799-6807 [Abstract] [Full Text]  
• Hintermann, E., Haake, S. K., Christen, U., Sharabi, A., Quaranta, V. (2002). Discrete Proteolysis of Focal Contact and Adherens Junction Components in Porphyromonas gingivalis-Infected Oral Keratinocytes: a Strategy for Cell Adhesion and Migration Disabling. Infect. Immun. 70: 5846-5856 [Abstract] [Full Text]