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Infection and Immunity, June 2002, p. 3304-3307, Vol. 70, No. 6
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.6.3304-3307.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Proteolysis of CD14 on Human Gingival Fibroblasts by Arginine-Specific Cysteine Proteinases from Porphyromonas gingivalis Leading to Down-Regulation of Lipopolysaccharide-Induced Interleukin-8 Production

Hiroyuki Tada,1,2 Shunji Sugawara,1* Eiji Nemoto,2 Nobuhiro Takahashi,3 Takahisa Imamura,4 Jan Potempa,5 James Travis,6 Hidetoshi Shimauchi,2 and Haruhiko Takada1

Department of Microbiology and Immunology,1 Department of Periodontics and Endodontics,2 Department of Oral Biochemistry, Tohoku University School of Dentistry, Sendai 980-8575,3 Division of Molecular Pathology, Department of Neuroscience and Immunology, Kumamoto University Graduate School of Medical Science, Kumamoto 860-0811, Japan,4 Department of Microbiology and Immunology, Institute of Molecular Biology, Jagiellonian University, 31-120 Craców, Poland,5 Department of Biochemistry, University of Georgia, Athens, Georgia 306026

Received 15 October 2001/ Returned for modification 11 December 2001/ Accepted 25 February 2002

Arginine-specific cysteine proteinases (gingipains-R) from periodontopathic Porphyromonas gingivalis cleaved CD14, a bacterial pattern recognition receptor, on human gingival fibroblasts (HGF). Consequently, gingipains-R reduced lipopolysaccharide-induced interleukin-8 production by HGF, indicating that gingipains-R inhibited CD14-dependent HGF activation and are involved in immune evasion by the bacterium in periodontal tissues.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Tohoku University School of Dentistry, 4-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan. Phone: 81-22-717-8306. Fax: 81-22-717-8309. E-mail: sugawars{at}mail.cc.tohoku.ac.jp.

Editor: J. D. Clements

Infection and Immunity, June 2002, p. 3304-3307, Vol. 70, No. 6
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.6.3304-3307.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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