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Infection and Immunity, July 2002, p. 3336-3343, Vol. 70, No. 7
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.7.3336-3343.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Identification of Chlamydia pneumoniae-Derived Mouse CD8 Epitopes

Anne Sarén,^1* Steve Pascolo,² Stefan Stevanovic,² Tilman Dumrese,^2, Mirja Puolakkainen,³ Matti Sarvas,¹ Hans-Georg Rammensee,² and Jenni M. Vuola¹

Department of Vaccines, National Public Health Institute,¹ Department of Virology, Haartman Institute and Helsinki University Central Hospital, University of Helsinki, Helsinki, Finland,³ Eberhard-Karls-Universität, Tübingen, Germany²

Received 25 January 2002/ Returned for modification 15 March 2002/ Accepted 18 April 2002

Chlamydia pneumoniae is a common intracellular human pathogen that has been associated with several severe pathological conditions, including coronary heart disease and atherosclerosis. There is no vaccine against C. pneumoniae infection, but CD8⁺ T cells have been shown to be crucial for protection during experimental infection. However, the effector functions and epitope specificity of the protective CD8⁺ T cell remain unknown. The aim of this study was to identify C. pneumoniae-derived mouse CD8 epitopes by using a recent epitope prediction method. Of four C. pneumoniae proteins (the major outer membrane protein, outer membrane protein 2, polymorphic outer membrane protein 5, and heat shock protein 60), 53 potential CD8⁺ T-cell epitopes were predicted by H-2 class I binding algorithms. Nineteen of the 53 peptides were identified as CD8 epitopes by testing for induction of a cytotoxic response after immunization. To test whether the predicted epitopes are naturally processed and presented by C. pneumoniae-infected cells, we generated a panel of seven peptide-specific cytotoxic T lymphocyte lines that were subsequently tested for recognition of C. pneumoniae-infected target cells. By using this strategy, we were able to identify three C. pneumoniae CD8 epitopes that were, indeed, processed and presented on infected cells. Identification of these natural CD8 epitopes provides tools for characterization of CD8⁺ T-cell function in vivo and generation of epitope-specific prevention strategies.

Editor: J. D. Clements

Present address: Institute of Experimental Immunology, University Hospital Zürich, Zürich, Switzerland.

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