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Infection and Immunity, July 2002, p. 3363-3370, Vol. 70, No. 7
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.7.3363-3370.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Modulation of C5aR Expression on Human Neutrophils by Encapsulated and Acapsular Cryptococcus neoformans

Claudia Monari,1 Thomas R. Kozel,2 Francesco Bistoni,1 and Anna Vecchiarelli1*

Microbiology Section, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, 06122 Perugia, Italy,1 Department of Microbiology, School of Medicine, University of Nevada, Reno, Nevada 89557-004622

Received 30 August 2001/ Returned for modification 30 October 2001/ Accepted 4 April 2002

Cryptococcus neoformans and cryptococcal surface polysaccharides influenced C5aR expression on human polymorphonuclear neutrophils (PMN). Encapsulated and acapsular strains produced dramatically different effects. Treatment of PMN with acapsular cryptococci up-regulated C5aR expression; whereas treatment with encapsulated cells suppressed C5aR expression. Glucuronoxylomannan (GXM), the principal constituent of the cryptococcal capsule, was responsible for such inhibition. Increased C5aR expression following treatment with acapsular cryptococci was accompanied by increased binding of C5a to PMN, increased superoxide production in response to stimulation with C5a, and an increased chemotactic response to C5a. Conversely, decreased C5aR expression following treatment with encapsulated cryptococci or acapsular cryptococci that had been pretreated with GXM was accompanied by decreased binding of C5a to PMN and a decreased chemotactic response to C5a. Our results raise the possibility that the down-regulation of C5aR expression by encapsulated cryptococci might alter PMN function at the site of cryptococcal infection.

* Corresponding author. Mailing address: Microbiology Section, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Via del Giochetto, 06122 Perugia, Italy. Phone: 39-075-585-7407. Fax: 39-075-585-7400. E-mail: vecchiar{at}unipg.it.

Editor: R. N. Moore

Infection and Immunity, July 2002, p. 3363-3370, Vol. 70, No. 7
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.7.3363-3370.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Monari, C., Pericolini, E., Bistoni, G., Casadevall, A., Kozel, T. R., Vecchiarelli, A. (2005). Cryptococcus neoformans Capsular Glucuronoxylomannan Induces Expression of Fas Ligand in Macrophages. J. Immunol. 174: 3461-3468 [Abstract] [Full Text]  
  • Ellerbroek, P. M., Lefeber, D. J., van Veghel, R., Scharringa, J., Brouwer, E., Gerwig, G. J., Janbon, G., Hoepelman, A. I. M., Coenjaerts, F. E. J. (2004). O-Acetylation of Cryptococcal Capsular Glucuronoxylomannan Is Essential for Interference with Neutrophil Migration. J. Immunol. 173: 7513-7520 [Abstract] [Full Text]  
  • Tissi, L., Puliti, M., Bistoni, F., Mosci, P., Kozel, T. R., Vecchiarelli, A. (2004). Glucuronoxylomannan, the Major Capsular Polysaccharide of Cryptococcus neoformans, Inhibits the Progression of Group B Streptococcal Arthritis. Infect. Immun. 72: 6367-6372 [Abstract] [Full Text]  


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